Molecular cloning and characterization of two zebrafish α(1,3)fucosyltransferase genes developmentally regulated in embryogenesis
Molecular cloning and characterization of two zebrafish α(1,3)fucosyltransferase genes developmentally regulated in embryogenesis
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DOI：
10.1093/oxfordjournals.jbchem.a022357
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发表时间：
1999-04-01
影响因子：
2.7
通讯作者：
Hase, S
中科院分区：
文献类型：
--
作者：
Kageyama, N;Natsuka, S;Hase, S
一些α(1,3)聚焦寡糖作为凝集素样粘附蛋白的对抗受体或在胚胎发生过程中具有时间精度表达，α(1,3)聚焦转移酶是这些寡糖产生的关键酶。从斑马鱼中克隆了两个α (1,3) focusyltransferase基因zFT1和zFT2，与其他基因的序列比较表明，zFT1和zFT2与人类α (1,3) focusyltransferase的氨基酸序列同源性约为30%。虽然迄今为止克隆的α (1,3) focusyltransferase根据氨基酸序列可分为三种类型——髓系、Lewis和白细胞，但系统发育分析表明，zFT1和zFT2都不属于这三种类型。哺乳动物细胞中zFT1或zFT2的表达可诱导α(1,3)聚焦转移酶活性，由吡啶胺化的乳-n -新四糖合成Lewis x结构，但乳-n -四糖不作为底物。逆转录-聚合酶链反应分析显示，zFT1在孵化前的有限时间内转录，而zFT2的mRNA仅在孵化后检测到。
Some alpha(1,3)fucosylated oligosaccharides serve as counter receptors to lectin-like adhesion proteins or are expressed with temporal precision during embryogenesis, and alpha(1,3)fucosyltransferase is a key enzyme in the production of these oligosaccharides. Two alpha(1,3)fucosyltransferase genes, designated zFT1 and zFT2, were cloned from zebrafish, Sequence comparisons with other genes indicated that zFT1 and zFT2 share about 30% amino acid sequence identity with human alpha(1,3)fucosyltransferases. Although the alpha(1,3)fucosyltransferases cloned so far can be classified into three types-myeloid, Lewis, and leukocyte-by virtue of their amino acid sequences, phylogenetic analysis indicated that neither zFT1 nor zFT2 belongs to any of these categories. The expression of zFT1 or zFT2 in mammalian cells induces alpha(1,3)fucosyltransferase activity to synthesize the Lewis x structure from pyridyl-aminated lacto-N-neotetraose however, lacto-N-tetraose does not serve as a substrate. Reverse transcriptase-polymerase chain reaction analysis revealed that zFT1 is transcribed during a restricted period before hatching, whereas the mRNA for zFT2 was detected only after hatching.