Parasympathetic inhibition of sympathetic neural activity to the pancreas
Parasympathetic inhibition of sympathetic neural activity to the pancreas
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DOI：
10.1152/ajpendo.2001.280.2.e378
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发表时间：
2001-02-01
影响因子：
5.1
通讯作者：
Taborsky, GJ
中科院分区：
文献类型：
--
作者：
Benthem, L;Mundinger, TO;Taborsky, GJ
本研究验证了副交感神经系统的激活可以减弱对胰腺的交感神经激活的假设。为了验证这一假设，我们测量了麻醉犬在双侧胸交感神经刺激（SNS; 8 Hz, 1 ms, 10 mA, 10 min）和不同时（随机设计）双侧颈迷走神经刺激（VNS; 8 Hz, 1 ms, 10 mA, 10 min）时胰腺去甲肾上腺素（NE）溢出（PNESO）。在刺激期间，仅在SNS期间，PNESO从基线的431 +/- 88 pg/ min增加到平均5137 +/- 1075 pg/ min （P < 0.05）。与单独使用SNS相比，同时使用SNS和VNS可显著（P < 0.01）降低PNESO反应[在刺激期内从411 +/- 61 pg/min降至平均2,760 +/- 1,005 pg/min (P < 0.05)]。SNS组动脉NE水平从130 +/- 11升高至600 pg/ml (P < 0.05)；同时使用SNS和VNS的反应明显小于（P < 0.05） （142 +/- 17 ~ 330 pg/ ml）。毒毒碱阻断不能阻止VNS降低PNESO或动脉NE对SNS反应的增加。结果表明，副交感神经活动不仅在胰岛水平上与交感神经活动相反，而且在神经水平上也与交感神经活动相反。这种神经抑制不是通过毒蕈碱机制介导的。
The present study tested the hypothesis that activation of the parasympathetic nervous system could attenuate sympathetic activation to the pancreas. To test this hypothesis, we measured pancreatic norepinephrine (NE) spillover (PNESO) in anesthetized dogs during bilateral thoracic sympathetic nerve stimulation (SNS; 8 Hz, 1 ms, 10 mA, 10 min) with and without (randomized design) simultaneous bilateral cervical vagal nerve stimulation (VNS; 8 Hz, 1 ms, 10 mA, 10 min). During SNS alone, PNESO increased from the baseline of 431 +/- 88 pg/ min to an average of 5,137 +/- 1,075 pg/ min (P < 0.05) over the stimulation period. Simultaneous SNS and VNS resulted in a significantly (P < 0.01) decreased PNESO response [from 411 +/- 61 to an average of 2,760 +/- 1,005 pg/min (P < 0.05) over the stimulation period], compared with SNS alone. Arterial NE levels increased during SNS alone from 130 +/- 11 to 600 pg/ml (P < 0.05); simultaneous SNS and VNS produced a significantly (P < 0.05) smaller response (142 +/- 17 to 330 pg/ ml). Muscarinic blockade could not prevent the effect of VNS from reducing the increase in PNESO or arterial NE in response to SNS. It is concluded that parasympathetic neural activity opposes sympathetic neural activity not only at the level of the islet but also at the level of the nerves. This neural inhibition is not mediated via muscarinic mechanisms.