Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers
Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers
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DOI：
10.1111/jfbc.13038
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发表时间：
2019-09-09
影响因子：
4
通讯作者：
Sarada, Ravi
中科院分区：
文献类型：
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作者：
Madhubalaji, Chegu Krishnamurthi;Rashmi, Venkatasubbaiah;Sarada, Ravi
通过调节实验动物中维生素B-12缺乏介导的生理和生化变化，螺旋藻被评价为维生素B-12的来源。用含螺旋藻饲料喂养B-12缺陷雄性断乳Wistar大鼠10周。尿甲基丙二酸增加（22.70 ± 4.08 μ mol/mol肌酐）和血浆同型半胱氨酸在B-12缺乏组中观察到（16.55 +/- 0.48 μ mol/L）水平，而在螺旋藻喂养组中这些水平与对照相等（肌酐分别为8.71 +/- 0.48 μ mol/mol和6.88 +/- 1.18 μ mol/L）。在螺旋藻喂养组中，血清（874.27 +/- 89.69）、血浆（615.53 +/- 26.5 pg/ml）、肾（10.19 +/- 1.066 ng/g）和肝组织（6.37 +/- 0.62 ng/g）中的维生素B-12水平与对照相似。如在B-12缺陷组中所见的睾丸中的严重萎缩性变化和肺和脾中的改变的组织结构在螺旋藻喂养组中正常化。本研究验证了螺旋藻能改善维生素B-12的状况。实际应用本研究表明，在维生素B-12缺乏大鼠的饮食中补充螺旋藻导致维生素B-12缺乏诱导的循环和功能生物标志物沿着生化和组织学变化的正常化。维生素B-12的素食来源是有限的，这里给出的结果为螺旋藻作为生物可利用维生素B-12的潜在素食来源的使用提供了科学验证。
Spirulina evaluated as a source of vitamin B-12 through the modulation of vitamin B-12 deficiency mediated physiological and biochemical changes in experimental animals. The B-12 deficient male weanling Wistar rats were fed with Spirulina-supplemented diet for 10 weeks. An increase in urinary methylmalonic acid (22.70 +/- 4.08 mu mol/moles of creatinine) and plasma homocysteine (16.55 +/- 0.48 mu mol/L) levels in the B-12 deficient group was observed, while these were equal to control in the Spirulina fed group (8.71 +/- 0.48 mu mol/mol of creatinine and 6.88 +/- 1.18 mu mol/L, respectively). The vitamin B-12 levels in serum (874.27 +/- 89.69), plasma (615.53 +/- 26.5 pg/ml), kidney (10.19 +/- 1.066 ng/g), and liver tissues (6.37 +/- 0.62 ng/g) in the Spirulina fed group were similar to control. Severe atrophic changes in the testes and altered tissue architecture in lung and spleen as seen in the B-12 deficient group were normalized in the Spirulina fed group. The study validates that Spirulina can improve the vitamin B-12 status. Practical applications The present study showed that the supplementation of Spirulina in the diet of vitamin B-12 deficient rats leads to the normalization of vitamin B-12 deficiency-induced circulatory and functional biomarkers along with biochemical and histological changes. Vegetarian sources for vitamin B-12 are limited and the results presented here provide scientific validation for the use of Spirulina as a potential vegetarian source of bioavailable vitamin B-12.