Design of hybrid optical tweezers system for automated 3D micro manipulation
Design of hybrid optical tweezers system for automated 3D micro manipulation
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用于自动化 3D 微操作的混合光镊系统设计
DOI：
10.1117/12.980463
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
et al.
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文献类型：
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作者：
Y. Tanaka;et al.
在我们以前的论文中，在动态处理大量的微珠阵列，我们开发了一个混合光镊系统，包括两个多光束技术：GPC方法和电流镜（GM）扫描方法。该系统具有高度的通用性，用于操纵大规模阵列，但由其GM扫描镊子形成的阵列只能在2.5维（2.5D）的工作空间中处理。这种限制是由于使用线性平台的透镜平移导致Z轴操纵的低带宽而产生的。对于真正的三维控制操作的多个微珠，在本文中，我们重新设计的GM扫描部分，以前的混合系统使用的电可调透镜具有高带宽。光学结构通过其落射荧光端口连接到市售显微镜。一组基于GPC的光镊使用p偏振光束，另一组基于GM扫描的光镊使用s偏振光束。在3D操作实验的结果中，演示了形成五边形的五个珠子和形成四面体的四个珠子围绕任意轴的受控旋转。
In our previous paper, on dynamic handling of massive micro-bead arrays, we developed a hybrid optical tweezers system consisting of two multi-beam techniques: the GPC method and the galvano mirrors (GMs) scanning method. This system had high versatility for manipulating massive arrays, but arrays formed by its GM scanning tweezers could be handled only in a two-and-half dimensional (2.5D) working space. This limitation arose from the low bandwidth of the Z-axis manipulation due to the lens translation using a linear stage. For true 3D controlled manipulation of multiple micro-beads, in this paper, we redesign the GM scanning part of the previous hybrid system using an electrically focus-tunable lens with high bandwidth. The optical structure is linked to a commercially available microscope via its epifluorescence port. One set of optical tweezers based on the GPC uses the p-polarized beam, and the other set based on the GM scanning uses the s-polarized beam. In the results of the 3D manipulation experiment, the controlled rotation of five beads forming a pentagon and that of four beads forming a tetrahedron about arbitrary axes are demonstrated.