Mycotoxins in poultry feed in Kuwait
Mycotoxins in poultry feed in Kuwait
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DOI：
10.1007/s00244-005-2094-0
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发表时间：
2006-05-01
影响因子：
4
通讯作者：
Saeed, T
中科院分区：
文献类型：
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作者：
Beg, MU;Al-Mutairi, M;Saeed, T
在科威特的一家家禽饲料生产单位进行了一项调查，以了解用作原料的黄玉米、大豆粉、麦麸样品以及为肉鸡开尾、肉鸡肥育和产蛋鸡准备的家禽饲料中的霉菌毒素污染情况。免疫亲和柱纯化后，通过高压液相色谱法测定单个黄曲霉毒素。重复分析显示玉米中的黄曲霉毒素平均浓度为0.27 ppb（范围0至1.69 ppb），豆粕0.20 ppb（范围0至1.27 ppb），麦麸0.15 ppb（范围0至1.07 ppb），0.48 ppb的肉鸡用预制家禽饲料（范围0至3.26 ppb），肉鸡肥育料为0.39 ppb（范围0至1.05 ppb），蛋鸡醪为0.21 ppb（范围0至1.30 ppb）。其他真菌毒素（赭曲霉毒素，伏马菌素，脱氧雪腐镰刀菌烯醇（DON），和玉米赤霉烯酮），通过定量酶联免疫吸附试验检测。在各类商品及配制饲料样本中，赭曲霉毒素A、伏马菌素、DON及玉米赤霉烯酮的平均含量分别为百万分之4.6至9.6、百万分之1.4至3.2、百万分之0.17至0.29及百万分之46.4至67.6。该研究显示，已测定的霉菌毒素共存，尽管其浓度一般低于家禽饲料的允许水平（无论定义如何）。
A survey was conducted at a poultry feed production unit in Kuwait for mycotoxin contamination in the samples of yellow maize, soybean meal, wheat bran used as raw material and the poultry feed prepared for broiler starter, broiler finisher, and layer mash. Individual aflatoxins were determined by high-pressure liquid chromatography after immunoaffinity column purification. Repeated analysis revealed average aflatoxin concentration in maize at 0.27 ppb (range 0 to 1.69 ppb), soybean meal at 0.20 ppb (range 0 to 1.27 ppb), wheat bran at 0.15 ppb (range 0 to 1.07 ppb), prepared poultry feed for broiler starter at 0.48 ppb (range 0 to 3.26 ppb), broiler finisher at 0.39 ppb (range 0 to 1.05 ppb), and layer mash at 0.21 ppb (range 0 to 1.30 ppb). Other mycotoxins (ochratoxin, fumonisin, deoxynivalenol (DON), and zearalenone), were detected by quantitative enzyme-linked immunosorbent assay. The average levels of ochratoxin A ranged from 4.6 to 9.6 ppb, fumonisin from 1.4 to 3.2 ppm, DON from 0.17 to 0.29 ppm, and zearalenone from 46.4 to 67.6 ppb in various commodities and prepared feed samples. The study revealed the coexistence of determined mycotoxins, although their concentrations in general were found to be lower than the permissible levels, wherever defined, for the poultry feed.