Iron loading into ferritin by an intracellular ferroxidase.
Iron loading into ferritin by an intracellular ferroxidase.
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通过细胞内亚铁氧化酶将铁加载到铁蛋白中。
DOI：
10.1006/abbi.1998.0891
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Aust,SD
中科院分区：
文献类型：
--
作者：
Reilly,CA;Aust,SD
一种细胞内的，膜结合的酶表现出对苯二胺氧化酶活性和亚铁氧化酶活性与马心的质膜部分分离，并研究其能力负载铁到铁蛋白。组织氧化酶的铁氧化酶活性被马脾脱铁蛋白刺激约两倍，铁被加载到铁蛋白中。抗马血清铜蓝蛋白抗体可抑制组织氧化酶将铁加载到铁蛋白中。组织来源的氧化酶和血清铜蓝蛋白将铁负载到铁蛋白中的过程中的铁氧化和氧消耗的化学计量分别为3.6 ± 0.2和3.9 ± 0.2。这些数据提供的证据表明，一种类似于血浆铜蓝蛋白的酶存在于马心脏的质膜上，并且这种铁氧化酶能够催化铁加载到铁蛋白中。这些数据的影响，目前的模型的吸收和储存的铁细胞进行了讨论。
An intracellular, membrane-bound enzyme exhibiting bothp-phenylenediamine oxidase activity and ferrous iron oxidase activity was isolated with the plasma membrane fraction of horse heart and studied for its ability to load iron into ferritin. The ferroxidase activity of the tissue oxidase was stimulated approximately twofold by horse spleen apoferritin, and the iron was loaded into ferritin. The loading of iron into ferritin by the tissue oxidase was inhibited by anti-horse serum ceruloplasmin antibody. The stoichiometry of iron oxidation and oxygen consumption during iron loading into ferritin by the tissue-derived oxidase and serum ceruloplasmin were 3.6 ± 0.2 and 3.9 ± 0.2, respectively. These data provide evidence that an enzyme analogous to ceruloplasmin is present on the plasma membrane of horse heart and that this ferroxidase is capable of catalyzing the loading of iron into ferritin. The implications of these data on the present models for the uptake and storage of iron by cells are discussed.