The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp.
The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp.
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DOI：
10.1038/s41598-018-30853-4
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发表时间：
2018-08-20
影响因子：
4.6
通讯作者：
Hoang TT
中科院分区：
文献类型：
--
作者：
Heacock-Kang Y;McMillan IA;Zarzycki-Siek J;Sun Z;Bluhm AP;Cabanas D;Hoang TT
天然能力需要从环境中摄取外源DNA，并且将该DNA整合到受体细菌中可用于DNA修复或遗传多样化。伯克霍尔德氏菌属是独特的，因为只有一些物种和菌株是天然的能力。我们从自然感受态B中鉴定并鉴定了两个基因，comE和crp。类鼻疽1026 b，其在DNA摄取和催化中起作用。在包括病原体B在内的非天然感受态伯克霍尔德氏菌中，鼠李糖诱导的comE和crp基因的单拷贝整合到Tn 7附着位点。pseudomallei K96243、B.新洋葱K56-2和B.鼻疽ATCC 23344。测定表达comE或crp的菌株摄取和分解代谢DNA的能力。ComE和Crp允许非天然感受态伯克霍尔德氏菌物种分解代谢DNA，摄取外源gfp DNA并表达GFP。此外，我们使用合成的comE和crp来扩展λ-red重组工程系统用于非感受态伯克霍尔德氏菌属物种的遗传操作的效用。用新构建的载体pKaKa 4突变4个B中的天冬氨酸半醛脱氢酶（asd）基因。鼻疽菌株，导致这些1级选择剂的完全衰减。这些菌株已被排除在选择剂法规之外，并且将引起该领域的极大兴趣。
Natural competency requires uptake of exogenous DNA from the environment and the integration of that DNA into recipient bacteria can be used for DNA-repair or genetic diversification. The Burkholderia genus is unique in that only some of the species and strains are naturally competent. We identified and characterized two genes, comE and crp, from naturally competent B. pseudomallei 1026b that play a role in DNA uptake and catabolism. Single-copies of rhamnose-inducible comE and crp genes were integrated into a Tn7 attachment-site in non-naturally competent Burkholderia including pathogens B. pseudomallei K96243, B. cenocepacia K56-2, and B. mallei ATCC23344. Strains expressing comE or crp were assayed for their ability to uptake and catabolize DNA. ComE and Crp allowed non-naturally competent Burkholderia species to catabolize DNA, uptake exogenous gfp DNA and express GFP. Furthermore, we used synthetic comE and crp to expand the utility of the λ-red recombineering system for genetic manipulation of non-competent Burkholderia species. A newly constructed vector, pKaKa4, was used to mutate the aspartate semialdehyde dehydrogenase (asd) gene in four B. mallei strains, leading to the complete attenuation of these tier-1 select-agents. These strains have been excluded from select-agent regulations and will be of great interest to the field.
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影响因子：
5
作者：
Corey, M;Farewell, V
通讯作者：
Farewell, V
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
4.4
作者：
Barrett, Ashley R.;Kang, Yun;Hoang, Tung T.
通讯作者：
Hoang, Tung T.
影响因子：
4.4
作者：
Kang, Yun;Norris, Michael H.;Hoang, Tung T.
通讯作者：
Hoang, Tung T.
DOI：
10.1073/pnas.1111020108
发表时间：
2011-10-11
影响因子：
11.1
作者：
Chantratita, Narisara;Rholl, Drew A.;Peacock, Sharon J.
通讯作者：
Peacock, Sharon J.