Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers.
Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers.
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DOI：
10.1251/bpo84
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发表时间：
2004-01-01
影响因子：
6.4
通讯作者：
George-Weinstein, Mindy
中科院分区：
文献类型：
--
作者：
Gerhart, Jacquelyn;Baytion, Michael;George-Weinstein, Mindy
用3DNA标记的树枝状聚合物进行原位杂交是检测组织切片和整个胚胎中低水平mRNA的一种新工具。荧光标记的树枝状聚合物用于鉴定早期鸡胚中表达骨骼肌转录因子MyoD mRNA的细胞。在原肠胚形成开始之前，在上胚层中发现了一小群MyoD mRNA阳性细胞，比先前使用酶或放射性标记的mRNA探针检测到的早两天。当从上胚层分离并置于培养物中时，MyoD mRNA阳性细胞能够分化为骨骼肌细胞。这些结果表明，DNA树状聚合物是用于鉴定单细胞和组织中低水平mRNA的灵敏和精确的工具。
In situ hybridization with 3DNA trade mark dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription factor MyoD in the early chick embryo. A small population of MyoD mRNA positive cells was found in the epiblast prior to the initiation of gastrulation, two days earlier than previously detected using enzymatic or radiolabeled probes for mRNA. When isolated from the epiblast and placed in culture, the MyoD mRNA positive cells were able to differentiate into skeletal muscle cells. These results demonstrate that DNA dendrimers are sensitive and precise tools for identifying low levels of mRNA in single cells and tissues.