Aqueous humour cytokine profiles after Descemet's membrane endothelial keratoplasty.
Aqueous humour cytokine profiles after Descemet's membrane endothelial keratoplasty.
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DOI：
10.1038/s41598-021-96566-3
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发表时间：
2021-08-23
影响因子：
4.6
通讯作者：
Yamagami S
中科院分区：
文献类型：
--
作者：
Hayashi T;Takahashi H;Inoda S;Shimizu T;Kobayashi A;Kawashima H;Yamaguchi T;Yamagami S
本研究的目的是比较大泡性角膜病变（BK）和年龄匹配的正常眼后弹力层内皮角膜移植术（DMEK）前后的房水（AqH）细胞因子谱。共49名参与者（平均年龄75.0 ± 13.5岁）被分为三组：（1）计划进行DMEK的BK患者（BK组）;（2）DMEK后的患者（DMEK组;平均术后时间1，018 ± 460天）;（3）计划进行白内障手术的无角膜内皮疾病的对照参与者（对照组）。从所有参与者收集AqH（0.2mL），并且使用多重珠免疫测定法同时测量各种AqH细胞因子的水平。与BK组相比，DMEK组显示出显著较低浓度的几种促炎细胞因子，如IL-1β、IL-5、IL-6、IL-10和IL-8以及粒细胞集落刺激因子。DMEK组IL-1β和IL-5水平明显低于对照组。DMEK后，几种促炎细胞因子的AqH水平显著降低，表明由于移植健康内皮而导致的内皮泵再生可能对前房炎症具有积极作用。
The aim of this study was to compare aqueous humour (AqH) cytokine profiles before and after Descemet’s membrane endothelial keratoplasty (DMEK) in eyes with bullous keratopathy (BK) and age-matched normal eyes. A total of 49 participants (mean age 75.0 ± 13.5 years) were divided into three groups: (1) BK patients scheduled for DMEK (BK group); (2) patients after DMEK (DMEK group; average postoperative time 1,018 ± 460 days); and (3) control participants without corneal endothelial disease scheduled for cataract surgery (control group). AqH (0.2 mL) was collected from all participants, and the levels of various AqH cytokines were simultaneously measured using a multiplex bead immunoassay. The DMEK group exhibited significantly lower concentrations of several pro-inflammatory cytokines, such as IL-1β, IL-5, IL-6, IL-10, and IL-8, and granulocyte colony stimulating factor than the BK group. In addition, the levels of IL-1β and IL-5 were significantly lower in the DMEK group than in the control group. The AqH levels of several pro-inflammatory cytokines were significantly reduced after DMEK, indicating that regeneration of the endothelial pump owing to the transplantation of a healthy endothelium might have a positive effect on anterior chamber inflammation.
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影响因子：
8.1
作者：
Monnereau, Claire;Quilendrino, Ruth;Melles, Gerrit R. J.
通讯作者：
Melles, Gerrit R. J.
影响因子：
13.7
作者：
Luznik, Zala;Oellerich, Silks;Dana, Reza
通讯作者：
Dana, Reza
影响因子：
2.8
作者：
Dapena, Isabel;Ham, Lisanne;Melles, Gerrit R. J.
通讯作者：
Melles, Gerrit R. J.
影响因子：
4.2
作者：
Ang, Marcus;Mehta, Jodhbir S.;Tan, Donald
通讯作者：
Tan, Donald
影响因子：
4.2
作者：
Hoerster, Robert;Stanzel, Tisha Prabriputaloong;Cursiefen, Claus
通讯作者：
Cursiefen, Claus