CD103+ Tumor Infiltrating Lymphocytes Predict a Favorable Prognosis in Urothelial Cell Carcinoma of the Bladder
CD103+ Tumor Infiltrating Lymphocytes Predict a Favorable Prognosis in Urothelial Cell Carcinoma of the Bladder
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CD103 肿瘤浸润淋巴细胞预测膀胱尿路上皮细胞癌的良好预后。
DOI：
10.1016/j.juro.2015.02.2941
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发表时间：
2015-08-01
影响因子：
6.6
通讯作者：
Huang, Jian
中科院分区：
文献类型：
--
作者：
Wang, Bo;Wu, Shaoxu;Huang, Jian
目的：不同肿瘤部位的 CD8(+) TIL 具有不同的临床属性，这可能是由于不同的肿瘤微环境促进分化为不同的亚群所致。然而，仅鉴定出少数可以定义 CD8(+) T 细胞亚群的标记。 CD103 是组织驻留记忆 CD8(+) T 细胞的标志物。在本回顾性研究中，我们探讨了膀胱尿路上皮细胞癌原位组织中CD103表达的细胞来源及其临床意义。材料与方法：采用免疫组化和免疫荧光技术鉴定膀胱尿路上皮细胞癌组织中CD103的细胞来源。应用Kaplan-Meier分析和Cox比例风险回归模型估计302例膀胱尿路上皮细胞癌患者的总生存期和无复发生存率。结果：膀胱尿路上皮细胞癌组织中CD103表达细胞以CD8(+) T细胞为主，而非自然杀伤细胞。值得注意的是，CD103(+) 细胞主要位于肿瘤内区域，而不是相关基质中 (p < 0.0001)。肿瘤内 CD103(+) TIL 的密度与肿瘤大小呈负相关 (p < 0.0001)，并且可以代表总生存期和无复发生存期的有利预后预测因子 (分别为 p = 0.002 和 0.011)。此外，肿瘤内CD103(+) TILs与膀胱尿路上皮细胞癌组织肿瘤内同源配体E-cadherin的表达呈正相关(p = 0.008)。结论：我们的研究结果表明，CD8(+) T细胞可能通过在膀胱尿路上皮细胞癌组织肿瘤内表达CD103而在肿瘤免疫中发挥重要作用。肿瘤内 CD103(+) TIL 可能作为膀胱尿路上皮细胞癌患者的预后标志物。
Purpose: CD8(+) TILs at different tumor sites have diverse clinical attributes, which might result from distinct tumor microenvironments that promote differentiation into distinct subsets. However, only a few markers have been identified that can define CD8(+) T-cell subsets. CD103 is a marker of tissue resident memory CD8(+) T cells. In this retrospective study we investigated the cellular source and clinical significance of CD103 expression in urothelial cell carcinoma of bladder tissues in situ.Materials and Methods: Immunohistochemistry and immunofluorescence were used to identify the cellular source of CD103 in bladder urothelial cell carcinoma tissues. Kaplan-Meier analysis and Cox proportional hazards regression models were applied to estimate overall and recurrence-free survival in 302 patients with bladder urothelial cell carcinoma.Results: CD8(+) T cells but not natural killer cells accounted for most CD103 expressing cells in bladder urothelial cell carcinoma tissues. Notably CD103(+) cells were predominantly located in intratumor regions rather than in associated stroma (p < 0.0001). The density of intratumor CD103(+) TILs was inversely associated with tumor size (p < 0.0001) and could represent a favorable prognostic predictor of overall and recurrence-free survival (p = 0.002 and 0.011, respectively). Moreover, intratumor CD103(+) TILs were positively associated with the expression of cognate ligand E-cadherin in intratumor regions of bladder urothelial cell carcinoma tissues (p = 0.008).Conclusions: Our findings suggest that CD8(+) T cells might have a significant role in tumor immunity by expressing CD103 in intratumor regions of bladder urothelial cell carcinoma tissues. Intratumor CD103(+) TILs could potentially serve as a prognostic marker in patients with bladder urothelial cell carcinoma.