Milk immunoglobulin with specific activity against purified colonization factor antigens can protect against oral challenge with enterotoxigenic Escherichia coli
Milk immunoglobulin with specific activity against purified colonization factor antigens can protect against oral challenge with enterotoxigenic Escherichia coli
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DOI：
10.1086/514227
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发表时间：
1998-03-01
影响因子：
6.4
通讯作者：
Crabb, JH
中科院分区：
文献类型：
--
作者：
Freedman, DJ;Tacket, CO;Crabb, JH
产肠毒素大肠杆菌（ETEC）是导致旅行者腹泻的最常见的分离病原体，也是发展中国家每年多达6.5亿例儿科腹泻的原因。作为预防性使用抗生素的安全替代品，开发了对纯化的定植因子抗原（CFA）具有特异性活性的超免疫牛乳抗体产品，并在人体挑战研究中进行了评价。25名健康成年志愿者经口接种10（9）cfu含CFA/I的ETEC。在随机双盲试验中，接受无乳糖安慰剂的10名志愿者中有7名在挑战后发生临床腹泻，而接受活性产品的志愿者中只有15名发生临床腹泻（Fisher精确检验，P <0.0017）。它的结论是，抗体对CFAs单独是足够的保护和预防与乳源性免疫球蛋白是一个可行的替代现有的药物干预。
Enterotoxigenic Escherichia coli (ETEC) is the most commonly isolated pathogen responsible for travelers' diarrhea and the cause of up to 650 million cases of pediatric diarrhea per year in the developing world. As a safe alternative to the prophylactic use of antibiotics, a hyperimmune bovine milk antibody product with specific activity against purified colonization factor antigens (CFAs) was developed and evaluated in a human challenge study. Twenty-five healthy adult volunteers were challenged orally with 10(9) cfu of a virulent CFA/I-bearing ETEC. In the randomized double-blind trial, 7 of 10 volunteers receiving a lactose-free placebo developed clinical diarrhea after challenge, compared with only 1 of 15 cases in volunteers receiving active product (Fisher's exact test, P < .0017). It is concluded that antibodies against CFAs alone are sufficient for protection and that prophylaxis with milk-derived immunoglobulin is a feasible alternative to existing drug interventions.