TXK, A NOVEL HUMAN TYROSINE KINASE EXPRESSED IN T-CELLS SHARES SEQUENCE IDENTITY WITH TEC FAMILY KINASES AND MAPS TO 4P12
TXK, A NOVEL HUMAN TYROSINE KINASE EXPRESSED IN T-CELLS SHARES SEQUENCE IDENTITY WITH TEC FAMILY KINASES AND MAPS TO 4P12
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DOI：
10.1093/hmg/3.6.897
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发表时间：
1994-06-01
影响因子：
3.5
通讯作者：
LITMAN, GW
中科院分区：
文献类型：
--
作者：
HAIRE, RN;OHTA, Y;LITMAN, GW
一种新的细胞质酪氨酸激酶TXK基因已经从人外周血的cDNA文库中分离出来。该基因的全序列分析表明，该基因与T细胞酪氨酸激酶EMT和人类X-连锁无球蛋白血症(XLA)和小鼠X-连锁免疫缺陷病(XID)中突变的B细胞酪氨酸激酶BTK密切相关。TXK和BTK一样，是Src型(非受体)酪氨酸激酶Tec亚家族的成员。与类似的Tec亚家族成员一样，TXK不同于其他的Src激酶，它既缺乏N端的肉豆蔻化信号，也缺乏C端的调节酪氨酸。TXK主要在T细胞和一些髓系细胞系中表达，但在许多其他类型的细胞中不表达。TXK与EMT和BTK在SH3、SH2和催化区有60%和57%的氨基酸同源性，但与BTK、EMT和TEE不同的是，它在N-末端缺乏Gap 1同源性和类固醇激素受体同源性。已分离出含有TXK的基因组克隆，并与染色体位置4p12杂交。
A gene for a novel, putative cytoplasmic tyrosine kinase, TXK has been isolated from a human peripheral blood cDNA library. The complete nucleotide sequence of the cDNA indicates that it is related mast closely to EMT, a tyrosine kinase of T cells and to the B-cell tyrosine kinase Btk, which is mutated in X-linked agammaglobulinemia (XLA) in humans and X-linked immunodeficiency disease (XID) in mouse. TXK, like BTK, is a member of the Tec sub-family of Src-type (non-receptor) tyrosine kinases. Like similar Tec subfamily members, and unlike the other Src kinases, TXK lacks both the N-terminal myristylation signal and the C-terminal regulatory tyrosine. TXK expression is detected primarily in T cells and some myeloid cell lines but not in a number of other cell types. TXK shares 60% amino acid homology with EMT and 57% with BTK over the SH3, SH2 (Src-homology) and catalytic domains but unlike BTK, EMT and tee, it lacks Gap 1 homology and steroid hormone receptor homology in the N-terminal region. Genomic clones containing TXK have been isolated and hybridize to chromosome position 4p12.