RELEASE OF MITOCHONDRIAL MATRIX PROTEINS THROUGH A CA-2+-REQUIRING, CYCLOSPORIN-SENSITIVE PATHWAY
RELEASE OF MITOCHONDRIAL MATRIX PROTEINS THROUGH A CA-2+-REQUIRING, CYCLOSPORIN-SENSITIVE PATHWAY
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DOI：
10.1016/0006-291x(89)92644-2
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发表时间：
1989-06-15
影响因子：
3.1
通讯作者：
PFEIFFER, DR
中科院分区：
文献类型：
--
作者：
IGBAVBOA, U;ZWIZINSKI, CW;PFEIFFER, DR
通常与小分子和离子从线粒体基质释放相关的内膜渗透性转变的诱导也引起基质蛋白的释放。释放与时间呈线性关系，与线粒体肿胀的时间过程相比，释放缓慢。高渗透性状态的瞬时诱导反映在蛋白质的瞬时释放中。环孢菌素A（0.5 nmol/mg蛋白质）或螯合游离Ca 2+，逆转渗透性转变，也阻止随后释放的蛋白质，即使在延长预孵育后加入。蛋白质释放的可能机制进行了讨论。
Induction of the inner membrane permeability transition, normally associated with the release of small molecules and ions from the mitochondrial matrix, also causes the release of matrix proteins. The release is linear with time and slow when compared to the time course of mitochondrial swelling. Transient induction of the high permeability state is reflected in transient release of proteins. Cyclosporin A (0.5 nmol/mg protein) or chelation of free Ca2+, which reverses the permeability transition, also block the subsequent release of protein even when added after extended preincubation. Possible mechanisms of protein release are discussed.