ISOLATION AND CHARACTERIZATION OF CHICKEN THYMIC ELECTROLECTIN
ISOLATION AND CHARACTERIZATION OF CHICKEN THYMIC ELECTROLECTIN
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DOI：
10.1042/bj2260379
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
TEICHBERG, VI
中科院分区：
文献类型：
--
作者：
LEVI, G;TEICHBERG, VI
β的存在-在成年鸡胸腺提取物中检测到D-半乳糖苷结合凝集素（电凝集素）。该凝集素通过在乳糖基-琼脂糖柱上的亲和层析纯化，从230 g胸腺中得到1.4 mg纯蛋白。当通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析时，鸡胸腺电凝集素（CTE）的MW为15，300，当通过凝胶过滤分析时，其MW为30，000。CTE的氨基酸组成与从人和大鼠肺中纯化的其他电凝集素的氨基酸组成相似。CTE与电鳗电器官和鸡胚胸肌的电凝集素有免疫交叉反应，但不完全相同。CTE凝集鸡胸腺细胞，但似乎并不促进其有丝分裂。
The presence of a .beta.-D-galactoside-binding lectin (electrolectin) was detected in extracts of the thymus of adult chickens. This lectin was purified by affinity chromatography on a lactosyl-Sepharose column to yield 1.4 mg of pure protein from 230g of thymus. The chicken thymic electrolectin (CTE) has MW of 15,300 when analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and of 30,000 when analyzed by gel filtration. The amino acid composition of CTE is similar to that of other electrolectins purified from human and rat lung. CTE crossreacts immunologically, but is not identical, with electrolectins from electric-eel electric organ and from chick-embryo pectoral muscle. CTE agglutinates chicken thymocytes but does not appear to promote their mitosis.