Analysis of erythropoiesis by erythroid colony formation in culture.
Analysis of erythropoiesis by erythroid colony formation in culture.
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通过培养中的红细胞集落形成来分析红细胞生成。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
N. Lin
中科院分区：
文献类型：
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作者：
Adamson Jw;B. Torok;N. Lin
造血祖细胞克隆测定的发展为分析正常和异常细胞调节提供了新工具。此类测定已应用于红细胞生成，并已证明前体细胞的类别在生长潜力、物理特征和对激素促红细胞生成素的敏感性方面存在显着差异。这些类别的正式基因学已经建立，并且通过贫血和红细胞增多动物的研究提供了对调节类别数量的因素的深入了解。结果表明，对培养物中可识别的最早的红系祖细胞的承诺不受促红细胞生成素控制，而最分化的元件则表明需要激素来维持它们。对控制红细胞分化的因素差异的认识预示着将发现多种机制来解释人类不同形式的红细胞衰竭。
The development of clonal assays for hemopoietic progenitors has provided new tools for the analysis of normal and abnormal cell regulation. Such assays have been applied to erythropoiesis and have demonstrated classes of precursors which differ significantly in their growth potential, physical characteristics, and sensitivity to the hormone, erythropoietin. A formal geneology of these classes has been established and insight into the factors that regulate the numbers of the classes provided by studies in anemic and polycythemic animals. The results suggest that commitment to the earliest erythroid progenitors recognizable in culture is not controlled by erythropoietin, whereas the most differentiated elements demonstrate a requirement for the hormone for their maintenance. Recognition of differences in the factors that control erythroid differentiation predict that a variety of mechanisms will be found to account for different forms of erythroid failure in man.