BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF THE KILLING OF HUMAN-MONOCYTES BY A LEUKOTOXIN DERIVED FROM ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF THE KILLING OF HUMAN-MONOCYTES BY A LEUKOTOXIN DERIVED FROM ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
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DOI：
10.1128/iai.28.1.258-268.1980
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
SANDERSON, CJ
中科院分区：
文献类型：
--
作者：
TAICHMAN, NS;DEAN, RT;SANDERSON, CJ
从伴生放线杆菌(菌株Y4)中提取了一种有效的、不稳定的白细胞毒性物质，放线菌是一种厌氧革兰氏阴性微生物，最初是从一名青少年牙周炎患者的龈下菌斑中分离出来的。用放射性胞浆标志物和细胞酶的胞外释放及延时显微摄影技术研究了Y4毒素对纯化的单核细胞的细胞病变效应。Y4毒素迅速结合到细胞上，产生剂量和时间依赖的变化，最终导致细胞死亡和细胞内成分释放到培养基中。单核细胞的细胞膜可能是这些现象发展的主要靶点。Y4毒素可杀死人多形核白细胞[PMN]，但不能杀死其他细胞类型。Y4毒素对牙周组织中中性粒细胞和单核细胞的破坏可能与青少年牙周炎的发病机制有关。
A potent, heat-labile leukotoxic material was extracted from A. actinomycetemcomitans (strain Y4), an anaerobic gram-negative microorganism originally isolated from subgingival plaque in a patient with juvenile periodontitis. The cytopathic effects of Y4 toxin on purified monocytes were studied by the extracellular release of radioactive cytoplasmic markers and cell enzymes and by time-lapse microcinematography. Y4 toxin rapidly bound to the cells, producing dose- and time-dependent alterations culminating in cell death and release of intracellular constituents into the culture medium. The cell membrane of the monocyte may be the primary target in the development of these phenomena. Y4 toxin kills human polymorphonuclear leukocytes [PMN] but not other cell types. Disruption of PMN and monocytes by Y4 toxin in the gingival crevice area may be relevant in the pathogenesis of juvenile periodontitis.