Norwalk-like virus 95/96-US strain is a major cause of gastroenteritis outbreaks in Australia
Norwalk-like virus 95/96-US strain is a major cause of gastroenteritis outbreaks in Australia
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DOI：
10.1002/jmv.10177
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发表时间：
2002-09-01
影响因子：
12.7
通讯作者：
Rawlinson, WD
中科院分区：
文献类型：
--
作者：
White, P;Hansman, G;Rawlinson, WD
诺沃克样病毒（NLV）的检测使用巢式逆转录酶-聚合酶链反应（RT-PCR）与针对RNA聚合酶区域的引物。在1997年至2000年期间，对11起单独爆发的胃肠炎和5起散发的儿童胃肠炎病例进行了样本检查。298 bp序列的系统发育分析表明，所有菌株都属于NLV基因组11，大多数测序的分离株（30/36）是1995年至1996年期间记录的全球爆发相关菌株的95/96-US子集的成员。这通过对代表性澳大利亚分离株的全长衣壳区的分析得到证实。本研究证明了NLV靶向引物对主要循环基因型的有用性，并证实了该亚型在全球（包括南半球）的传播。(C)2002 Wiley-Liss，Inc.
Norwalk-like viruses (NLVs) were detected using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) with primers directed to the RNA polymerase region. Samples were examined from 11 separate outbreaks of gastroenteritis and five sporadic cases of childhood gastroenteritis between 1997 and 2000. Phylogenetic analysis of the 298 bp sequences showed that all strains belong to NLV genogroup 11 and the majority of the sequenced isolates (30/36) were members of the 95/96-US subset of strains associated with outbreaks recorded worldwide between 1995 and 1996. This was confirmed by analysis of the full length capsid region of a representative Australian isolate. This study demonstrates the usefulness of targeting primers for NLVs to the predominant circulating genotype(s) and confirms the spread of this subtype globally, including the Southern Hemisphere. (C) 2002 Wiley-Liss, Inc.