Immunohistochemical reactivity of myometrial oxytocin receptor in extracorporeally perfused nonpregnant human uteri
Immunohistochemical reactivity of myometrial oxytocin receptor in extracorporeally perfused nonpregnant human uteri
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DOI：
10.1007/s00404-003-0474-0
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发表时间：
2003-03
影响因子：
2.6
通讯作者：
O. Richter;K. Tschubel;J. Schmolling;M. Kupka;U. Ulrich;E. Wardelmann
中科院分区：
文献类型：
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作者：
O. Richter;K. Tschubel;J. Schmolling;M. Kupka;U. Ulrich;E. Wardelmann
在妊娠子宫内，催产素和催产素受体对子宫收缩和引产起着重要作用。临床证据表明，对于与收缩性相关的疾病，比如痛经也发生在未怀孕和孕早期的子宫肌层催产素和催产素受体似乎比目前认为的更重要。然而，人们对催产素受体、催产素和17-β-雌二醇在非妊娠子宫肌层中的相互依赖性以及催产素受体在非妊娠子宫中的分布知之甚少。因此，在本研究中，我们研究了17-β-雌二醇和催产素刺激是否会影响催产素受体在非妊娠子宫肌层的表达。方法采用已建立的实验性子宫灌注系统。在无17-β-雌二醇（A组，n=5）或17-β-雌二醇高刺激（B组，n=5）的生理条件下灌注10只子宫27 h，两组在实验最后3 h均进行催产素刺激。用免疫组织化学方法比较两组子宫内膜催产素受体的表达。结果与阴性对照相比，免疫组化反应显示子宫底催产素受体浓度升高，且在17-β-雌二醇和催产素刺激下子宫底催产素受体浓度最高（40%阳性染色细胞，p<0.01）。然而，催产素受体水平没有达到与妊娠晚期标本相当的浓度，后者被用作阳性对照。综上所述，我们的数据表明，17-β-雌二醇和催产素的刺激不仅可以影响妊娠子宫，也可以影响非妊娠子宫的催产素受体表达动态。因此，非妊娠期子宫肌层的收缩异常现象也可能通过17-β-雌二醇、催产素和催产素受体介导。
IntroductionIn the pregnant uterus oxytocin and the oxytocin receptor play a major part for uterine contractility and the induction of labor. Clinical evidence implicates that with regard to contractility associated disorders like for example dysmenorrhea also in the nonpregnant and very early pregnant myometrium oxytocin and the oxytocin receptor seem to be more important than believed at the moment. However, little is known about the mutual dependence of the oxytocin receptor, oxytocin and 17-β-estradiol in the nonpregnant myometrium and about the distribution of the oxytocin receptor in the nonpregnant uterus. Therefore, in the present study we investigated in the nonpregnant myometrium if oxytocin receptor expression can be affected by 17-β-estradiol and oxytocin stimulation.MethodsWe used a previously established experimental perfusion system for the human uterus. We perfused 10 uteri for 27 h under physiological conditions without 17-β-estradiol (group A,n=5) or with high 17-β-estradiol stimulation (group B,n=5) followed by oxytocin stimulation in both groups in the last 3 h of the experiment. The expression of the myometrial oxytocin receptor in both groups was compared immunohistochemically.ResultsIn comparison to the negative controls the immunohistochemical reactivity demonstrated increasing oxytocin receptor concentrations with maximum levels under 17-β-estradiol and oxytocin stimulation in the uterine fundus (40% of positive stained cells,p<0.01). However, oxytocin receptor levels did not reach concentrations comparable to specimen of third trimester of pregnancy, which were used as positive controls.ConclusionsTaken together, our data demonstrate that the dynamics of oxytocin receptor expression can be affected by stimulation with 17-β-estradiol and oxytocin not only in the pregnant uterus, but also in the nonpregnant uterus. Therefore, dyscontractile phenomena of the nonpregnant myometrium also may be mediated via 17-β-estradiol, oxytocin and the oxytocin receptor.