PiQSi: Protein quaternary structure investigation
PiQSi: Protein quaternary structure investigation
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DOI：
10.1016/j.str.2007.09.019
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发表时间：
2007-11-01
期刊：
影响因子：
5.7
通讯作者：
Levy, Emmanuel D.
中科院分区：
文献类型：
--
作者：
Levy, Emmanuel D.
PiQSi有助于在蛋白质数据库（PDB）中手动研究蛋白质复合物的四级结构。用户可以浏览并获得给定蛋白质及其进化亲属的四级结构信息的概述，这有助于确定生物四级状态。我已经使用这个框架注释了PDB生物单元中的10，000多个结构，并纠正了其中15%的类似四级状态。一个基准测试表明，注释是高质量的，并强调需要手动策展，特别是对于模棱两可的情况下，如蛋白质在两个四元状态之间的平衡。数据库中已有的近似10，000个注释可用于提高蛋白质结构分析的准确性或预测蛋白质四级结构的基准方法。此外，PiQSi还集成了一个基于社区的策展系统，我希望这将使我们能够准确而完整地描述PDB中蛋白质的生物四级状态。PiQSi可在http://www.PiQSi.org/访问。
PiQSi facilitates the manual investigation of the quaternary structure of protein complexes in the Protein Data Bank (PDB). Users can browse and obtain an overview of the quaternary structure information of a given protein together with its evolutionary relatives, which helps in the determination of the biological quaternary state. I have used this framework to annotate over 10,000 structures from the PDB Biological Unit and corrected the quaternary state of similar to 15% of them. A benchmark shows that the annotations are of high quality and stresses the need for manual curation, in particular for ambiguous cases such as proteins in equilibrium between two quaternary states. The similar to 10,000 annotations already in the database can be used to improve the accuracy of analyses on protein structure or to benchmark methods that predict protein quaternary structure. In addition, PiQSi incorporates a community-based curation system, which I hope will allow us to reach an accurate and complete description of the biological quaternary state of proteins in PDB. PiQSi is accessible at http://www.PiQSi.org/.