The course of tissue permeabilization studied on a mathematical model of a subcutaneous tumor in small animals
The course of tissue permeabilization studied on a mathematical model of a subcutaneous tumor in small animals
复制标题
DOI：
10.1109/tbme.2005.851524
复制
发表时间：
2005-08-01
影响因子：
4.6
通讯作者：
Miklavcic, D
中科院分区：
文献类型：
--
作者：
Pavselj, N;Bregar, Z;Miklavcic, D
增强化疗药物抗肿瘤效果的方法之一是局部应用短强电脉冲。这导致细胞膜通透性增加，称为电透化。为了研究小动物皮下肿瘤的组织透化过程，采用有限元方法，用商业程序EMAS建立了数学模型。该模型是基于在文献中发现的组织比电导率值，实验确定的电场阈值的可逆和不可逆的组织透化，和组织中的电导率变化。然后将用该模型获得的结果与来自小鼠皮下肿瘤治疗的实验结果进行比较，并且获得了良好的一致性。我们的研究结果和可逆和不可逆的阈值使用的电化学疗法在真实的肿瘤中的有效性吻合良好，其中实验表明，在高于900 V/cm比率的幅度下具有抗肿瘤效果，并且在1300 V/cm比率下具有显著的抗肿瘤效果。
One of the ways to potentiate antitumor effectiveness of chemotherapeutic drugs is by local application of short intense electric pulses. This causes an increase of the cell membrane permeability and is called electropermeabilization. In order to study the course of tissue permeabilization of a subcutaneous tumor in small animals, a mathematical model was built with the commercial program EMAS, which uses the finite element method. The model is based on the tissue specific conductivity values found in literature, experimentally determined electric field threshold values of reversible and irreversible tissue permeabilization, and conductivity changes in the tissues. The results obtained with the model were then compared to experimental results from the treatment of subcutaneous tumors in mice and a good agreement was obtained. Our results and the reversible and irreversible thresholds used coincide well with the effectiveness of the electrochemotherapy in real tumors where experiments show antitumor effectiveness for amplitudes higher than 900 V/cm ratio and pronounced antitumor effects at 1300 V/cm ratio.