Molecular analysis of the genome segments S1, S4, S6, S7 and S12 of a Rice gall dwarf virus isolate from Thailand;: completion of the genomic sequence
Molecular analysis of the genome segments S1, S4, S6, S7 and S12 of a Rice gall dwarf virus isolate from Thailand;: completion of the genomic sequence
复制标题
DOI：
10.1007/s00705-007-0948-7
复制
发表时间：
2007-07-01
影响因子：
2.7
通讯作者：
Omura, T.
中科院分区：
文献类型：
--
作者：
Moriyasu, Y.;Maruyama-Funatsuki, W.;Omura, T.
测定了泰国稻瘿矮病毒（Rice gall dwarf virus， RGDV）基因组S1、S4、S6、S7和S12双链RNA片段的全核苷酸序列。这些片段由4505、2622、1648、1652和853个核苷酸组成，编码1458、725、489、511和206个氨基酸，分子质量分别约为166、80、53、59和24 kDa。同源性研究表明，每一种假定的蛋白在植物呼肠孤病毒属的另外两种水稻矮病毒（RDV）和伤口肿瘤病毒（Wound tumor virus）的分离物中都有对应的蛋白。然而，没有发现与其他已登记序列的相似性，包括属于呼肠孤病毒科的其他病毒的序列。RGDV与RDV同源结构蛋白的同源性在34 ~ 51%之间，高于RGDV与RDV同源非结构蛋白的同源性（16 ~ 37%）。在非结构蛋白中，RGDV Pns11和RDV Pns10的氨基酸序列一致性最高（37%）。RGDV Pns11和RDV Pns10是管状包涵体的组成部分。这一观察结果表明，一种特定的氨基酸主链可能不仅需要维持病毒粒子的三维结构，而且还需要维持包涵体的三维结构。RGDV基因组的整个序列现在是可用的。
The complete nucleotide sequences of the double-stranded RNA segments S1, S4, S6, S7 and S12 of the genome of a Rice gall dwarf virus (RGDV) isolate from Thailand were determined. The segments consisted of 4505, 2622, 1648, 1652 and 853 nucleotides, encoding putative proteins of 1458, 725, 489, 511 and 206 amino acids with molecular masses of approximately 166, 80, 53, 59 and 24 kDa, respectively. Homology searches indicated that each of the putative proteins has a counterpart in isolates of Rice dwarf virus (RDV) and Wound tumor virus, two other species in the genus Phytoreovirus. However, no similarities were found to other registered sequences, including those of other viruses that belong to the family Reoviridae. The identities between homologous structural proteins of RGDV and RDV ranged from 34 to 51% and were thus higher than those between homologous non-structural proteins of RGDV and RDV (16-37%). Among the nonstructural proteins, the highest amino acid sequence identity (37%) was observed for RGDV Pns11 and RDV Pns10, a constituent of tubular inclusions. This observation suggests that a specific amino acid backbone might be required for maintaining not only the three-dimensional structure of virions but also that of inclusions. The entire sequence of the RGDV genome is now available.