Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
Genistein diet improves body weight, serum glucose and triglyceride levels in both male and female ob/ob mice
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DOI：
10.2147/dmso.s216312
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Al-Nakkash, Layla
中科院分区：
文献类型：
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作者：
Rockwood, Schuyler;Mason, Daniel;Al-Nakkash, Layla
目的：瘦素缺陷ob/ob小鼠的糖尿病肥胖与体重增加、高血糖和高胰岛素血症有关。我们之前已经研究了染料木素(一种天然存在于大豆中的异黄酮)对肥胖/肥胖小鼠代谢紊乱的影响，并证明了染料木素(600 mg/kg饮食，连续4周)对T-3产生和皮质酮状态的有益影响。这项研究的目的是检验饮食金雀异黄素是否可以预防或至少减少糖尿病肥胖小鼠模型中的典型表型，并评估潜在的性别差异。患者和方法：4-5周龄的ob/ob小鼠(雄性和雌性)被随机分配到两种饮食中的一种，为期4周：标准啮齿动物饮食或含金雀异黄素的饮食(600 mg金雀异黄素/公斤饮食)。结果：金雀异黄素饮食显著降低了女性体重12%和男性体重9%。金雀异黄素显著降低女性和男性的血糖水平18%和43%，但对血清胰岛素没有影响。金雀异黄素饮食显着降低肥胖/肥胖雄性和雌性小鼠的血清甘油三酯水平，使它们恢复到瘦肉水平。仅在女性中，金雀异黄素显著降低血清胰多肽水平56%，增加血清GIP水平2.3倍。金雀异黄素对肝脏脂肪变性有性别依赖性的影响：在雌性，金雀异黄素使脂肪面积百分比和脂滴直径进一步增加2.6倍，并增加肝脏总胆红素。结论：本研究结果表明金雀异黄素饮食对雌雄ob/ob小鼠都有有趣的有益效果。
Purpose: Diabetic obesity in the leptin-deficient ob/ob mouse is associated with weight gain, and hyperglycemia, along with hyperinsulinemia. We have previously examined the effects of genistein (a naturally occurring isoflavone found in soy) on metabolic disturbances in the ob/ob mouse and demonstrated beneficial effects of genistein (600 mg genistein/kg diet, for 4-weeks) on T-3 production and corticosterone status. The goal of this study was to examine whether dietary genistein could prevent, or at least lessen, the typical phenotype in this murine model of diabetic-obesity, and to assess potential sex-differences.Patients and methods: The ob/ob mice (male and female) aged 4-5 weeks were randomly assigned to one of two diets for a period of 4-weeks: standard rodent diet, or genistein-containing diet (600 mg genistein/kg diet). Comparisons were made to a lean control group.Results: Genistein diet significantly reduced body weight by 12% in females and 9% in males. Genistein significantly lowered serum glucose levels by 18% in females and 43% in males, yet had no effect on serum insulin. Genistein diet significantly lowered serum triglyceride levels in both ob/ob male and female mice returning them to lean levels. In females only, genistein significantly reduced serum pancreatic polypeptide levels by 56% and increased serum GIP levels 2.3-fold. Genistein had sex-dependent effects on hepatic steatosis: in females, genistein further increased the % fat area and the fat droplet diameter 2.6-fold, along with additionally increasing hepatic TBARS.Conclusion: The results from this study indicate interesting beneficial effects of genistein diet for both male and female ob/ob mice.