Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin
Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin
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DOI：
10.3390/polym10080812
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发表时间：
2018-07
期刊：
影响因子：
5
通讯作者：
Kentaro Yoshida;Katsuhiko Sato;Tetsuya Ono;Takenori Dairaku;Yoshitomo Kashiwagi
中科院分区：
文献类型：
--
作者：
Kentaro Yoshida;Katsuhiko Sato;Tetsuya Ono;Takenori Dairaku;Yoshitomo Kashiwagi
采用钠离子（NAF）和聚丙烯胺盐酸盐（PAH）、聚乙基亚胺（PEI）、聚二烯基二甲基氯化铵（PDDA）等聚阳离子（LbL）逐层沉积法制备薄膜。然后胰岛素通过浸入胰岛素溶液被吸附在nafl -polycation LbL膜上。利用石英晶体微天平和原子力显微镜对naff -多阳离子LbL薄膜进行了表征。利用紫外可见吸附光谱和荧光发射光谱对胰岛素在LbL膜中的释放进行了表征。胰岛素在NAF-PAH LbL膜上的吸附量最大。（NAF/PAH）5NAF LbL膜在1 mg mL−1的胰岛素溶液中（pH 7.4）吸附的胰岛素量为61.8µg cm−2。在pH 2.0和pH 9.0的胰岛素溶液中，LbL膜的胰岛素释放量高于pH 7.4。因此，naff -polycation可以在温和条件下作为胰岛素递送LbL膜，也可以根据pH变化作为胰岛素释放控制系统。
Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), and poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL films by immersion in an insulin solution. The NAF-polycation LbL films were characterized using a quartz crystal microbalance and an atomic force microscope. The release of insulin from the LbL films was characterized using UV-visible adsorption spectroscopy and fluorescence emission spectroscopy. The greatest amount of insulin was adsorbed on the NAF-PAH LbL film. The amount of insulin adsorbed on the (NAF/PAH)5NAF LbL films by immersion in a 1 mg mL−1 insulin solution at pH 7.4 was 61.8 µg cm−2. The amount of insulin released from the LbL films was higher when immersed in insulin solutions at pH 2.0 and pH 9.0 than at pH 7.4. Therefore, NAF-polycations could be employed as insulin delivery LbL films under mild conditions and as an insulin release control system according to pH change.