Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds.
Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds.
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DOI：
10.1371/journal.pone.0079307
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Yang Y;Wei F;Zhuo R;Fan F;Liu H;Zhang C;Ma L;Jiang M;Zhang X
漆酶可用于各种生物技术和工业应用。白腐菌Trametesvelutina 5930及其漆酶是本实验室从神农架自然保护区分离到的一株具有环境生物学应用潜力的真菌。然而，在没有任何诱导剂的情况下，毛栓菌5930产生的漆酶的初始水平相对较低。因此，为了提高绒栓菌5930漆酶的产量，使该菌在环境生物技术领域得到更好的利用，本研究对绒栓菌5930漆酶的产生和漆酶基因的表达调控进行了研究。不同的金属离子如Cu ~（2+）和Fe ~（2+）对绒栓菌漆酶合成和漆酶基因转录均有不同程度的促进作用。一些与木质素结构相关的芳香族化合物，如单宁酸、胡萝卜酸、肉桂酸、没食子酸和愈创木酚等，也能提高漆酶活性和漆酶基因转录水平。研究还发现，芳香族化合物和金属离子对绒栓菌漆酶的产生和漆酶基因的转录具有协同效应。综上所述，本研究为绒栓菌5930漆酶产酶能力的提高提供了理论依据。
Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu2+ and Fe2+ could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.
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影响因子：
5.3
作者：
THIELE, DJ
通讯作者：
THIELE, DJ
DOI：
10.1073/pnas.87.10.3826
发表时间：
1990-05-01
影响因子：
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作者：
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影响因子：
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作者：
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Cambria, Antonio