Ecl1 is a zinc binding protein that is involved in the zinc-limitation dependent extension of chronological lifespan in fission yeast.
Ecl1 is a zinc binding protein that is involved in the zinc-limitation dependent extension of chronological lifespan in fission yeast.
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Ecl1 是一种锌结合蛋白，参与裂殖酵母中锌限制依赖性延长时间寿命。
DOI：
10.1007/s00438-016-1285-x
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发表时间：
2017
影响因子：
3.1
通讯作者：
Hirofumi Aiba
中科院分区：
文献类型：
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作者：
Takafumi Shimasaki;Hokuto Ohtsuka;Chikako Naito;Takeshi Tenno;Hidekazu Hiroaki;Hiroshi Murakami;Hirofumi Aiba
过表达ecl1家族基因（ecl1+、ecl2+和ecl3+）可延长裂糖菌的按时间顺序排列的寿命。然而，这种扩展的机制还没有明确定义。ecl1家族蛋白由大约80个氨基酸组成，其中有4个半胱氨酸残基在它们的n端结构域中是保守的。本研究聚焦于Ecl1蛋白，顺序突变其半胱氨酸残基以确认其重要性。结果，所有突变的Ecl1蛋白几乎失去了延长时间寿命的功能，这表明这四种半胱氨酸残基对Ecl1蛋白至关重要。利用电感耦合等离子体原子发射光谱（ICP-AES）分析，我们发现野生型Ecl1蛋白含有锌，而半胱氨酸突变的Ecl1蛋白不含锌。我们还分析了环境锌对时间顺序寿命的影响。我们发现，锌的限制延长了按时间顺序排列的寿命，这种延长取决于ecl1家族蛋白。
Overexpression of Ecl1-family genes (ecl1+,ecl2+, andecl3+) results in the extension of the chronological life span inSchizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein. Utilizing ICP-AES (inductively coupled plasma atomic emission spectroscopy) analysis, we found that wild-type Ecl1 proteins contain zinc, while cysteine-mutated Ecl1 proteins do not. We also analyzed the effect of environmental zinc on the chronological life span. We found that zinc limitation extends the chronological life span, and this extension depends on the Ecl1-family proteins.