THE DROSOPHILA SEGMENTATION GENE RUNT ENCODES A NOVEL NUCLEAR REGULATORY PROTEIN THAT IS ALSO EXPRESSED IN THE DEVELOPING NERVOUS-SYSTEM
THE DROSOPHILA SEGMENTATION GENE RUNT ENCODES A NOVEL NUCLEAR REGULATORY PROTEIN THAT IS ALSO EXPRESSED IN THE DEVELOPING NERVOUS-SYSTEM
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DOI：
10.1101/gad.4.10.1701
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发表时间：
1990-10-01
影响因子：
10.5
通讯作者：
GERGEN, JP
中科院分区：
文献类型：
--
作者：
KANIA, MA;BONNER, AS;GERGEN, JP
果蝇胚胎的前后体模式的产生需要分割基因的活性。片段基因runt被归类为主要的配对规则基因之一，因为它在调节其他配对规则基因的表达方面发挥着关键作用。在这里，我们介绍了该基因的结构，并描述了矮小蛋白在胚胎发育过程中的表达模式。推导出的蛋白质序列与数据库中的任何序列都没有明显的整体同源性。由于缺乏可识别的转录因子基序(如同源异型盒、锌指、亮氨酸拉链或螺旋-环-螺旋)，使得矮小蛋白不同于其他早期作用的片段蛋白。产生了一种矮小的特异性多克隆抗体，并用来证明该蛋白的亚细胞位置在细胞核内。通过双染色免疫定位实验确定矮小蛋白与配对规则基因Haily(H)、Even-Skip(Eve)和FushTarazu(FTZ)的重叠。我们发现矮小和有毛的图案是互补的。它们的相位相对于互补的EVE和FTZ图案向前移动两个晶胞直径。对矮小抗体的实验还表明，该蛋白在整个胚胎发育过程中都存在，并在发育中的中枢和周围神经系统中广泛表达。
Generation of the anterior-posterior body pattern in the Drosophila embryo requires the activity of the segmentation genes. The segmentation gene runt has been classified as one of the primary pair-rule genes because of the pivotal role it plays in regulating the expression of other pair-rule genes. Here, we present the structure of this gene and describe the pattern of runt protein expression during embryogenesis. The deduced protein sequence shows no obvious overall homology with any sequences in the data base. The absence of an identifiable transcription factor motif (e.g., homeo box, zinc finger, leucine zipper, or helix-loop-helix) makes runt different from the other early-acting segmentation proteins. A runt-specific polyclonal antibody was generated and used to demonstrate that the subcellular location of the protein is in the nucleus. Double-staining immunolocalization experiments were used to determine the overlap of the runt protein pattern with the patterns of the pair-rule genes hairy (h), even-skipped (eve), and fushi tarazu (ftz). We found that the patterns of runt and hairy are complementary. Their phasing is shifted anteriorly by two cell diameters with respect to the complementary eve and ftz patterns. Experiments with the runt antibody also indicated that the protein is present throughout embryogenesis and is expressed extensively in the developing central and peripheral nervous system.