Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by oridonin nanosuspension
Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by oridonin nanosuspension
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DOI：
10.3109/10717544.2010.536271
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发表时间：
2011-05-01
期刊：
影响因子：
6
通讯作者：
Zhang, Qiang
中科院分区：
文献类型：
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作者：
Feng, Fei-Fei;Zhang, Dian-Rui;Zhang, Qiang
以MCF-7人乳腺癌细胞为模型，研究了高压匀浆法制备的冬凌草甲素（ORI）纳米混悬液的体外抗肿瘤作用机制。MTT法、形态学观察、流式细胞仪分析和western blot分析表明，与ORI溶液相比，ORI纳米混悬液能显著增强MCF-7细胞的体外抗肿瘤活性。此外，ORI纳米混悬液诱导MCF-7细胞的G（2）/M期增殖停滞和凋亡取决于其浓度。Western blot分析显示，ORI纳米悬浮液处理的细胞中，caspase-3前体蛋白未被切割成活化形式，抗凋亡Bcl-2蛋白表达降低，而促凋亡Bax蛋白表达呈剂量依赖性增加。这些观察结果表明，ORI纳米混悬液的抗肿瘤活性通过细胞周期阻滞和细胞凋亡诱导而增强。
The mechanism for anti-tumor activity of oridonin (ORI) nanosuspension, prepared by the high pressure homogenization method, was studied using MCF-7 human breast carcinoma cells in vitro. MTT assay, observation of morphologic changes, flow cytometric analysis, and western blot analysis indicated that ORI nanosuspension could significantly intensify the in vitro anti-tumor activity to MCF-7 cells, as compared with ORI solution. Furthermore, ORI nanosuspension induced G(2)/M stage proliferation arrest and apoptosis in MCF-7 cells depending on its concentration. In addition, western blot analysis indicated that the pro-caspase-3 protein was not cleaved into the activated form and the expression of anti-apoptotic Bcl-2 protein decreased, on the contrary, the expression of pro-apoptotic Bax protein increased in a dose-dependent manner in ORI nanosuspension-treated cells. These observations indicated that the anti-tumor activity of ORI nanosuspension was intensified by cell-cycle arrest and apoptosis induction.