Disrupted cortical map and absence of cortical barrels in growth-associated protein (GAP)-43 knockout mice
Disrupted cortical map and absence of cortical barrels in growth-associated protein (GAP)-43 knockout mice
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DOI：
10.1073/pnas.96.16.9397
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发表时间：
1999-08-03
影响因子：
11.1
通讯作者：
Meiri, KF
中科院分区：
文献类型：
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作者：
Maier, DL;Mani, S;Meiri, KF
有强有力的证据表明，生长相关蛋白（GAP-43），一种仅在神经系统中发现的蛋白质，调节神经元对轴突导向信号的反应。然而，它在大脑皮层复杂空间模式中的作用尚未被探索。我们发现，缺乏GAP-43表达（-/-）的小鼠未能建立有序的晶须表示（桶阵列）通常发现在啮齿动物初级体感皮层的第四层。丘脑皮质传入-/-皮质形成不规则的补丁层IV内定义不清的皮质领域，这是不同的半球，而不是刻板的，胡须特异性，隔离地图中看到的正常动物。此外，许多丘脑皮层传入项目异常广泛分离的皮质目标。总之，我们的研究结果表明，差距-43 -/-小鼠皮层中可识别的须领土的损失，在这里，我们提出了一个中断的体细胞定位图表型在皮层中，在其他无桶突变体中的有序的须地图内的边界模糊形成鲜明对比。我们的研究结果表明，GAP-43的表达是至关重要的正常建立有序的地形在桶皮质。
There is strong evidence that growth-associated protein (GAP-43), a protein found only in the nervous system, regulates the response of neurons to axonal guidance signals. However, its role in complex spatial patterning in cerebral cortex has not been explored. We show that mice lacking GAP-43 expression (-/-) fail to establish the ordered whisker representation (barrel array) normally found in layer IV of rodent primary somatosensory cortex. Thalamocortical afferents to -/- cortex form irregular patches in layer IV within a poorly defined cortical field, which varies between hemispheres, rather than the stereotypic, whisker-specific, segregated map seen in normal animals. Furthermore, many thalamocortical afferents project abnormally to widely separated cortical targets. Taken together, our findings indicate a loss of identifiable whisker territories in the GAP-43 -/- mouse cortex, Here, we present a disrupted somatotopic map phenotype in cortex, in clear contrast to the blurring of boundaries within an ordered whisker map in other barrelless mutants. Our results indicate that GAP-43 expression is critical for the normal establishment of ordered topography in barrel cortex.