EXPRESSION OF FOLLICLE-STIMULATING-HORMONE RECEPTOR MESSENGER-RNA IN RAT TESTES AND SERTOLI CELLS
EXPRESSION OF FOLLICLE-STIMULATING-HORMONE RECEPTOR MESSENGER-RNA IN RAT TESTES AND SERTOLI CELLS
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DOI：
10.1210/mend-5-5-670
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发表时间：
1991-05-01
影响因子：
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通讯作者：
GRISWOLD, MD
中科院分区：
文献类型：
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作者：
HECKERT, LL;GRISWOLD, MD
为了更好地了解FSH在生殖中的作用，我们对其受体在雄性大鼠体内的表达进行了研究。通过聚合酶链式反应制备了大鼠卵泡刺激素受体(FSHR)的DNA探针，并分离到部分基因组克隆。Northern印迹分析显示，FSHR有两个转录本，一个是2.6kb的转录本，它是主要的mRNA，另一个是4.5kb的转录本。这两个转录本是睾丸和卵巢特异的，在睾丸中，该基因的表达似乎主要在支持细胞中。Northern印迹分析表明，FSHR mRNA在10-60日龄大鼠的睾丸以及培养的20日龄和成年动物的支持细胞中均有表达。Northern印迹分析表明，与生精上皮特定阶段同步的FSHR基因在第XIII-II期中表达最高，在第VII-VIII期最低。在生精上皮周期中，FSHR的mRNA水平发生了3倍以上的变化。
In order to better understand the role of FSH in reproduction, we have studied the expression of its receptor in the male rat. A DNA probe for the rat FSH receptor (FSHR) was generated by the polymerase chain reaction, and a partial genomic clone was isolated. Northern blot analysis revealed two transcripts for the FSHR, a 2.6-kilobase (kb) transcript, which is the predominant mRNA, and a 4.5-kb transcript. The two transcripts were specific to the testis and ovary, and in the testis, the expression of the mRNA appeared to be primarily in the Sertoli cells. Northern blot analysis showed the presence of FSHR mRNA in testes from rats ranging in age from 10-60 days as well as in Sertoli cells cultured from 20-day-old and adult animals. FSHR mRNA levels in testes synchronized to specific stages of the seminiferous epithelium were shown by Northern blot analysis to be highest in stages XIII-II and to decrease to a minimum at stages VII-VIII. The levels of FSHR mRNA underwent more than a 3-fold change during the cycle of the seminiferous epithelium.