Cell kinetic measurements in prostate cancer
Cell kinetic measurements in prostate cancer
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DOI：
10.1016/s0360-3016(96)00579-2
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发表时间：
1997-03-15
影响因子：
7
通讯作者：
Fowler, JF
中科院分区：
文献类型：
--
作者：
Haustermans, KMG;Hofland, I;Fowler, JF
目的：建议采用两种方法来增加前列腺癌放射治疗的总剂量。一是适形放射治疗；另一种是使用许多小分数的超分割。两者都意味着总治疗时间可能会延长。为了判断延长治疗方案是否有害，有必要了解人类前列腺肿瘤的增殖率，特别是潜在倍增时间（Tpot）。文献中缺乏关于该参数的数据。方法和材料：对七名前列腺腺癌患者进行了研究。在进行活检之前4-12小时静脉内输注100 mg/m(2) IUdR示踪剂量。将活检组织固定在 70% 乙醇中，保存在 4°C 下，随后通过细胞计数的标准方法进行制备和染色，使用 DNA 的红色荧光信号和 5-碘-2'-脱氧尿苷的绿色荧光信号（异硫氰酸荧光素）。 DNA 合成的持续时间 (Ts) 通过相对运动 (RM) 方法确定，已知示踪剂施用和活检之间的间隔。 Tpot 计算为 Ts 除以标记指数 (LI) 的商。结果：七个肿瘤中有两个 LI 太低（
Purpose: Two approaches have been suggested for escalating the total dose in radiotherapy treatment of prostate cancer. One is conformal radiotherapy; the other is hyperfractionation using many small fractions. Both imply some possible prolongation in overall treatment time. To judge whether prolonged treatment schedules would be detrimental, it is necessary to know the proliferation rates in human prostate tumors, specifically, the potential doubling time (Tpot). There is a lack of data on this parameter in the literature.Methods and Materials: Seven patients with adenocarcinoma of the prostate were studied. A tracer dose of 100 mg/m(2) of IUdR was infused intravenously 4-12 h before biopies were taken. Biopsies were fixed in 70% ethanol, stored at 4 degrees C, and later prepared and stained by standard methods for how cytometry, using the red fluorescence signal for DNA and the green fluorescence signal (fluorescein isothiocyanate) for 5-iodo-2'-deoxyuridine. The duration of DNA synthesis (Ts) was determined by the relative movement (RM) method, knowing the interval between tracer administration and biopsy. Tpot was calculated as the quotient of Ts by labeling index (LI).Results: In two of the seven tumors the LI was too low (