Contrast angiography of the rat renal microcirculation in vivo using synchrotron radiation
Contrast angiography of the rat renal microcirculation in vivo using synchrotron radiation
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DOI：
10.1152/ajprenal.90499.2008
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发表时间：
2009-05-01
影响因子：
4.2
通讯作者：
Pearson, James T.
中科院分区：
文献类型：
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作者：
Eppel, Gabriela A.;Lo Jacono, David;Pearson, James T.
EPPEL GA，Jacono DL，Shirai M，Umetani K，Evans RG，Pearson JT。同步辐射活体大鼠肾微循环对比血管造影术。Am J Physiol Renal Physiol 296：F1023-F1031,2009。2009年3月4日首次出版；DOI：10.1152/ajprenal.90499.2008。-我们开发了一种使用同步辐射对大鼠肾循环进行微血管造影术的新方法。应用该方法测定肾动脉血管对血管紧张素II和电刺激肾神经的反应。在戊巴比妥钠麻醉大鼠静脉注射血管紧张素Ⅱ(1.6 mU·kg~(-1)·min~(-1))或其载体，或2)RNS(频率为2赫兹)之前和期间，将碘化造影剂直接注入肾动脉。在这些治疗前和治疗期间，以30赫兹获得图像，并通过使用在此描述的新开发的算法来确定血管口径。沿动脉树可同时观察到多达4个水平的分支，由28-400微米的静息直径组成。血管内径不受载体输注(+3.1+/-3.5%改变)的显著改变，但被血管紧张素II(-24.3+/-3.4%)和RNS(-17.1+/-3.8%)显著缩小。血管紧张素II引起的血管收缩与血管大小无关，但RNS引起的血管收缩在静息口径为100~200微米的血管中最大，在静息口径为40~100微米的血管中最小。
Eppel GA, Jacono DL, Shirai M, Umetani K, Evans RG, Pearson JT. Contrast angiography of the rat renal microcirculation in vivo using synchrotron radiation. Am J Physiol Renal Physiol 296: F1023-F1031, 2009. First published March 4, 2009; doi:10.1152/ajprenal.90499.2008.-We have developed a new method for contrast microangiography of the rat renal circulation using synchrotron radiation. The method was applied to determine responses of the renal arterial vasculature to angiotensin II and electrical stimulation of the renal nerves ( RNS). Iodinated contrast agent was administered directly into the renal artery of pentobarbital-anesthetized rats before and during 1) intravenous infusion of angiotensin II (1.6 mu g.kg(-1).min(-1)) or 2) its vehicle, or 3) RNS at 2 Hz. Images were obtained at 30 Hz, before and during these treatments, and vascular caliber was determined by use of a newly developed algorithm described herein. Up to four levels of branching could be observed simultaneously along the arterial tree, comprising vessels with resting diameter of 28-400 mu m. Vessel diameter was not significantly altered by vehicle infusion (+3.1 +/- 3.5% change) but was significantly reduced by angiotensin II (-24.3 +/- 3.4%) and RNS (-17.1 +/- 3.8%). Angiotensin II-induced vasoconstriction was independent of vessel size, but RNS-induced vasoconstriction was greatest in vessels with a resting caliber of 100-200 mu m and least in vessels with a resting caliber 40-100 mu m. In conclusion, the method we describe herein provides a new approach for assessing responses of the renal arterial circulation to vasoactive factors along several orders of branching.