Flow-cytometric monitoring of intracellular flavins simultaneously with NAD(P)H levels.
Flow-cytometric monitoring of intracellular flavins simultaneously with NAD(P)H levels.
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流式细胞术同时监测细胞内黄素和 NAD(P)H 水平。
DOI：
10.1002/cyto.990040109
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
B. Thorell
中科院分区：
文献类型：
--
作者：
B. Thorell
描述了一种细胞荧光仪，使用氩紫外（351-363 nm）和氩蓝（488 nm）激光的组合。这种双激发可以同时监测黄素和NAD(P)H的氧化还原状态，作为细胞代谢状态的指示物。光散射、吸收和外源性荧光染料染色可以增加额外的信息。因此，五参数流分析成为可能。本文报道了分离的大鼠肝细胞和小鼠骨髓中黄素和NAD(P)H的测定。
A cytofluorimeter is described, using the combination of Argon UV (351-363 nm) and Argon Blue (488 nm) lasers. The dual excitation makes it possible to monitor simultaneously the redox state of flavins and NAD(P)H as indicative of cell metabolic state. Light scatter, absorption, and staining with exogenous fluorescent dyes can add additional information. Thus, a five-parameter flow analysis becomes possible. The present paper describes flavin and NAD(P)H measurements on isolated rat liver cells and mouse bone marrow.