Identification of genes encoding putative nucleoporins and transport factors in the fission yeast Schizosaccharomyces pombe:: a deletion analysis
Identification of genes encoding putative nucleoporins and transport factors in the fission yeast Schizosaccharomyces pombe:: a deletion analysis
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DOI：
10.1002/yea.1115
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发表时间：
2004-04-30
期刊：
影响因子：
2.6
通讯作者：
Balasundaram, D
中科院分区：
文献类型：
--
作者：
Chen, XQ;Du, XM;Balasundaram, D
在研究裂殖酵母裂殖酵母中与核细胞质运输相关的基因的系统方法中，删除了 26 个假定的核孔蛋白和运输因子的开放阅读框 (ORF)。在这里，我们报告了这些缺失突变体的初步表征。在 26 个假定被删除的基因中，有 14 个被发现对于生存至关重要。必需基因的无效突变导致无法完成一轮或无法维持细胞分裂。 14 个必需基因中的 4 个，SPBC582.11c、SPBC17G9.04c、SPBC3B9.16c 和 SPCC162.08c，分别编码推定的核孔蛋白和肌球蛋白样蛋白，其同源物 NUP84、NUP85、NUP120 和 MLP1，这些基因对于酿酒酵母的生存能力不是必需的，这表明它们的基因产物在 Sz 中执行关键功能。庞贝。在结合药物敏感性测定和遗传分析的基础上，我们鉴定了五个非必需无效突变体，它们对微管解聚药物噻菌灵 (TBZ) 过敏，并在 TBZ 治疗后表现出剪切表型，表明可能参与微管功能。三个相应的 ORF，SPCC18B5.07c、nup40 和 SPAC1805.04，分别编码与酿酒酵母核孔蛋白 NUP2p、NUP53p 和 NUP133p 具有较低相似性的推定核孔蛋白。进一步的遗传分析表明，编码假定的 importin-beta/Cse1p 超家族蛋白的一个核孔蛋白基因 nup40 和另一个基因 SPCC1322.06 可能具有纺锤体检查点功能。版权所有 (C) 2004 John Wiley Sons, Ltd.
In a systematic approach to study genes that are related to nucleocytoplasmic trafficking in the fission yeast Schizosaccharomyces pombe, the open reading frames (ORFs) of 26 putative nucleoporins and transport factors were deleted. Here we report the initial characterization of these deletion mutants. Of the 26 putative genes deleted, 14 were found to be essential for viability. Null mutations of essential genes resulted in failure to either complete one round or to sustain cell division. Four of the 14 essential genes, SPBC582.11c, SPBC17G9.04c, SPBC3B9.16c and SPCC162.08c, encode putative nucleoporins and a myosin-like protein with homologues NUP84, NUP85, NUP120 and MLP1, respectively, that are not required for viability in Saccharomyces cerevisiae, suggesting that their gene products perform critical functions in Sz. pombe. On the basis of combined drug sensitivity assays and genetic analysis we have identified five non-essential null mutants that were hypersensitive to the microtubule depolymerizing drug thiabendazole (TBZ) and exhibited a cut phenotype upon TBZ treatment, suggesting possible involvement in microtubule function. Three of the corresponding ORFs, SPCC18B5.07c, nup40 and SPAC1805.04, encode putative nucleoporins with low similarity to the S. cerevisiae nucleoporins NUP2p, NUP53p and NUP133p, respectively. Further genetic analysis revealed that one of the nucleoporin genes, nup40, and another gene, SPCC1322.06, encoding a putative importin-beta/Cse1p superfamily protein may have a spindle checkpoint function. Copyright (C) 2004 John Wiley Sons, Ltd.