Transcriptional profiling and miRNA-dependent regulatory network analysis of longissimus dorsi muscle during prenatal and adult stages in two distinct pig breeds.
Transcriptional profiling and miRNA-dependent regulatory network analysis of longissimus dorsi muscle during prenatal and adult stages in two distinct pig breeds.
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DOI：
10.1111/age.12032
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发表时间：
2013-08
期刊：
影响因子：
2.4
通讯作者：
P. Siengdee;N. Trakooljul;E. Muráni;M. Schwerin;K. Wimmers;S. Ponsuksili
中科院分区：
文献类型：
--
作者：
P. Siengdee;N. Trakooljul;E. Muráni;M. Schwerin;K. Wimmers;S. Ponsuksili
microRNAs（miRNAs）和mRNAs建立了一个复杂的调控网络，影响包括肌肉发育和生长在内的多种生物学途径。阐明参与肌肉发育的miRNA依赖性调控网络可以为产前发育期间预先定义的肌肉性状提供额外的见解。本研究的目的是确定差异表达的转录本和功能miRNA-mRNA的关系与骨骼肌发育的不同阶段，在两个猪品种，德国长白猪和皮特兰，不同的肌肉特性。使用Affyphase GeneChip猪基因组微阵列对来自妊娠后35、63和91天的胎儿以及成年猪（出生后180天）的背最长肌组织进行比较转录谱分析。差异表达模式被确定为与肌肉发育阶段和品种类型。整合miRNA表达数据和独创性途径分析（ipa）途径分析揭示了几个与肌肉生长和发育相关的miRNA依赖性调控网络。本研究结果为进一步改善猪肉品质提供了肌肉生物学的见解。
MicroRNAs (miRNAs) and mRNAs establish a complex regulatory network influencing diverse biological pathways including muscle development and growth. Elucidating miRNA-dependent regulatory networks involved in muscle development could provide additional insights into muscle traits largely predefined during prenatal development. The present study aimed to determine differentially expressed transcripts and functional miRNA-mRNA relationships associated with different stages of skeletal muscle development in two pig breeds, German Landrace and Pietrain, distinct in muscle characteristics. A comparative transcriptional profiling of longissimus dorsi muscle tissues from fetuses at 35, 63 and 91 days post-conception as well as adult pigs (180 days postnatum) was performed using the Affymetrix GeneChip porcine genome microarray. Differential expression patterns were identified to be associated with muscularly developmental stages and breed types. The integration of miRNA expression data and ingenuity pathways analysis (ipa) pathway analysis revealed several miRNA-dependent regulatory networks related to muscle growth and development. The present results provide insights into muscle biology for further improvement of porcine meat quality.