Application of the Improved BALB/c 3T3 Cell Transformation Assay to the Examination of the Initiating and Promoting Activities of Chemicals: The Second Inter-laboratory Collaborative Study by the Non-genotoxic Carcinogen Study Group of Japan
Application of the Improved BALB/c 3T3 Cell Transformation Assay to the Examination of the Initiating and Promoting Activities of Chemicals: The Second Inter-laboratory Collaborative Study by the Non-genotoxic Carcinogen Study Group of Japan
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改进的 BALB/c 3T3 细胞转化测定法在化学物质引发和促进活性检测中的应用：日本非遗传毒性致癌物研究组的第二次实验室间合作研究
DOI：
10.1177/026119291003800111
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
T. Yatsushiro
中科院分区：
文献类型：
--
作者：
T. Tsuchiya;M. Umeda;N. Tanaka;A. Sakai;Hiroshi Nishiyama;I. Yoshimura;S. Ajimi;Shin Asada;M. Asakura;H. Baba;Yasuaki Dewa;Youji Ebe;Y. Fushiwaki;Yuji Hagiwara;S. Hamada;Tetsuo Hamamura;Y. Iwase;Y. Kajiwara;Y. Kasahara;Y. Kato;M. Kawabata;Emiko Kitada;K. Kaneko;Yuko Kizaki;Kinya Kubo;D. Miura;K. Mashiko;F. Mizuhashi;D. Muramatsu;M. Nakajima;Tetsu Nakamura;H. Oishi;Toshiaki Sasaki;Sawako Shimada;C. Takahashi;Y. Takeda;S. Wakuri;N. Yajima;S. Yajima;T. Yatsushiro
日本环境诱变剂协会的非基因毒性致癌物研究小组组织了利用BALB/c 3T3细胞进行一期和两期细胞转化试验的实验室间合作研究的第二步，目的是确认各自实验室是否能够独立产生与起始或促进相关的结果。在细胞生长的固定阶段，采用添加2%胎牛血清的DMEM/F12培养基和胰岛素、转铁蛋白、乙醇胺和亚硒酸钠的混合物。17个实验室参与了这项研究，每种化学物质都由3到5个实验室进行测试。一级和二级分析的比较表明，后一种方法将有利于筛选化学品。在两阶段法启动活性试验中（后处理0.1μg/ml 12- o-十四醇-13-乙酸酯），相关检测实验室苯并[a]芘和甲烷磺酸甲酯检测结果均为阳性，菲检测结果为阴性。在指定将非那西丁用于起始阶段的实验室中，两个实验室的检测结果为阳性，两个实验室的检测结果为阴性，后者的检测剂量比前者使用的最大剂量低一个剂量。在两阶段法（以0.2μg/ml 3-甲基胆蒽预处理）促活性的探索中，相关检测实验室对甲泽林、原钒酸钠、TGF-β1检测结果为阳性，对蒽醌、非那西丁、佛波检测结果为阴性。12,13-二癸酸福波尔的两个结果为阳性，但一个结果为阴性——同样，达到后者结果的最大剂量低于产生前者结果的最大剂量。这些结果表明，只要充分考虑剂量问题，如最大剂量的确定，这种改进的分析方法是相关的、可重复的和可转移的。建议其他研究小组和监管当局考虑应用这种两阶段分析来筛选化学品的启动和促进潜力。
The Non-genotoxic Carcinogen Study Group in the Environmental Mutagen Society of Japan organised the second step of the inter-laboratory collaborative study on one-stage and two-stage cell transformation assays employing BALB/c 3T3 cells, with the objective of confirming whether the respective laboratories could independently produce results relevant to initiation or promotion. The method was modified to use a medium consisting of DMEM/F12 supplemented with 2% fetal bovine serum and a mixture of insulin, transferrin, ethanolamine and sodium selenite, at the stationary phase of cell growth. Seventeen laboratories collaborated in this study, and each chemical was tested by three to five laboratories. Comparison between the one-stage and two-stage assays revealed that the latter method would be beneficial in the screening of chemicals. In the test for initiating activity with the two-stage assay (post-treated with 0.1μg/ml 12-O-tetradecanoylphorbol-13-acetate), the relevant test laboratories all obtained positive results for benzo[a]pyrene and methylmethane sulphonate, and negative results for phenanthrene. Of those laboratories assigned phenacetin for the initiation phase, two returned positive results and two returned negative results, where the latter laboratories tested up to one dose lower than the maximum dose used by the former laboratories. In the exploration of promoting activity with the two-stage assay (pretreated with 0.2μg/ml 3-methylcholanthrene), the relevant test laboratories obtained positive results for mezerein, sodium orthovanadate and TGF-β1, and negative results for anthralin, phenacetin and phorbol. Two results returned for phorbol 12,13-didecanoate were positive, but one result was negative — again, the maximum dose to achieve the latter result was lower than that which produced the former results. These results suggest that this modified assay method is relevant, reproducible and transferable, provided that dosing issues, such as the determination of the maximum dose, are adequately considered. The application of this two-stage assay for screening the initiating and promoting potential of chemicals is recommended for consideration by other research groups and regulatory authorities.
转化生长因子β对小鼠上皮JB6细胞贴壁独立生长的影响。
影响因子：
11.2
作者：
Wilder,PJ;Rizzino,A
通讯作者：
Rizzino,A
影响因子：
11.2
作者：
S. Patierno;N. Lehman;B. Henderson;J. Landolph
通讯作者：
S. Patierno;N. Lehman;B. Henderson;J. Landolph
BALB/c-3T3 细胞的转化：V. 168 种化学物质的转化反应与沙门氏菌的致突变性和啮齿动物生物测定中的致癌性相比。
影响因子：
10.4
作者：
Matthews,EJ;Spalding,JW;Tennant,RW
通讯作者：
Tennant,RW