Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB), a neural member of the EGF family of differentiation factors, is implicated in neurite formation.
Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB), a neural member of the EGF family of differentiation factors, is implicated in neurite formation.
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DOI：
10.1083/jcb.136.4.895
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发表时间：
1997-02-24
期刊：
影响因子：
--
通讯作者：
Rathjen FG
中科院分区：
文献类型：
--
作者：
Schumacher S;Volkmer H;Buck F;Otto A;Tarnók A;Roth S;Rathjen FG
通过将鸡神经系统中的结合测定与免疫筛选相结合，将鸡酸性富含 EGF 样结构域的脑蛋白 (CALEB) 鉴定为 EGF 分化因子家族的新成员。 cDNA 克隆表明 CALEB 是一种多结构域蛋白，由 NH2 末端糖基化区域、富含亮氨酸-脯氨酸的片段、酸性盒、单个 EGF 样结构域、跨膜和短胞质延伸组成。在发育中的神经系统中，CALEB 与神经胶质和神经元表面相关。 CALEB 由 140/130 kD 双峰、80 kD 带和含硫酸软骨素的 200 kD 组分组成。后两种成分在胚胎神经系统中表达，并在成人神经系统中下调。 CALEB 与细胞外基质糖蛋白生腱蛋白-C 和 -R 结合。体外抗体扰动实验揭示了 CALEB 在许可环境中参与神经突形成。
Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB) was identified by combining binding assays with immunological screens in the chicken nervous system as a novel member of the EGF family of differentiation factors. cDNA cloning indicates that CALEB is a multidomain protein that consists of an NH2-terminal glycosylation region, a leucine-proline–rich segment, an acidic box, a single EGF-like domain, a transmembrane, and a short cytoplasmic stretch. In the developing nervous system, CALEB is associated with glial and neuronal surfaces. CALEB is composed of a 140/130-kD doublet, an 80-kD band, and a chondroitinsulfate-containing 200-kD component. The latter two components are expressed in the embryonic nervous system and are downregulated in the adult nervous system. CALEB binds to the extracellular matrix glycoproteins tenascin-C and -R. In vitro antibody perturbation experiments reveal a participation of CALEB in neurite formation in a permissive environment.