Protein architecture and the origin of introns.
Protein architecture and the origin of introns.
复制标题
蛋白质结构和内含子的起源。
DOI：
10.1101/sqb.1987.052.01.100
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
M. Nosaka
中科院分区：
文献类型：
--
作者：
M. Go;M. Nosaka
方法最初引入的模块是通过将一个球状结构域划分成由约20-40个连续氨基酸残基组成的相对紧凑的区域而定义的片段（G6 1981）。该算法被应用于小蛋白质，如溶菌酶（G6 1983）和细胞色素c（G6 1985），一个小的结构域，如卵类粘蛋白第三结构域（G5 1985），和一个小的亚基，血红蛋白α和β链（G5 1981）。在这些没有核心模块埋在内部的单层结构域或亚单元中，模块的接头的特征在于它们的位置不靠近结构域或亚单元的表面（G6 1981）。利用这一特点以及紧凑性本身使得更容易识别的模块在一个小域或亚基组成的单层模块;模块可以识别的事实，他们的关节位于不远处的所有其他残基。搜索这样的关节进行了使用距离图上的氨基酸残基彼此分开超过一定的距离被标记。
METHODModules introduced originally are segments defined by partitioning a globular domain into relatively compact regions consisting of about 20-40 contiguous amino acid residues (G6 1981). The algorithm was applied to a small protein such as lysozyme (G6 1983) and cytochrome c (G6 1985), a small domain such as ovomucoid third domain (G5 1985), and a small subunit, hemoglobin a and/3 chains (G5 1981). In these monolayer domains or subunits having no core modules buried inside, the joints of the modules are characterized by their locations not being close to the surface of the domains or subunits (G6 1981). Utilization of this characteristic as well as the compactness itself makes easier the identification of modules in a small domain or subunit consisting of monolayer modules; the modules can be identified by the fact that their joints are located not far from all the other residues. Search for such joints was carried out by using a distance map on which amino acid residues separated from each other by more than a certain distance were marked.