Comparison of Transplantation of Lung Organoid Cell Types: One Size Does Not Fit All.
Comparison of Transplantation of Lung Organoid Cell Types: One Size Does Not Fit All.
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DOI：
10.1165/rcmb.2021-0263le
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发表时间：
2022-03
影响因子：
6.4
通讯作者：
E. Ararat;S. M. Louie;E. Lu;Margherita Paschini;David M. Raiser;Carla F. Kim
中科院分区：
文献类型：
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作者：
E. Ararat;S. M. Louie;E. Lu;Margherita Paschini;David M. Raiser;Carla F. Kim
图1。不同细胞群移植的比较。(A)实验设计示意图。(B)代表性的DsRed小鼠用于准备移植细胞或器官培养的流式细胞术选通。DAPI 2，DsRed 1细胞的三个群体的门控策略是彩色编码的：红色，CD45 2，CD31 2细胞；蓝色，CD45 2，CD31 2，EpCAM 1，SCA1 2细胞；绿色，CD45 2，CD31 2，EPCAM 1，SCA1 1细胞。(C)用流式细胞仪对受体小鼠肺进行图形分析。总共50-100万个新分选的CD31 2/45 2细胞或新分选的细胞
Figure 1. Comparison of transplantation of different cell populations.(A) Schematic of experiment design.(B) Representative flow cytometry gating from DsRed mouse in preparation of cells for transplant or organoid culture. Gating strategy is color-coded for three populations from the DAPI 2, DsRed 1 cells: red, CD45 2, CD31 2 cells; blue, CD45 2, CD31 2, EpCAM 1, SCA1 2 cells; green, CD45 2, CD31 2, EpCAM 1, SCA1 1 cells.(C) Graphical analysis of recipient mouse lungs by flow cytometry. A total of 0.5–1 million freshly sorted CD31 2/45 2 cells or freshly sorted