Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis
Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis
复制标题
胚胎发生过程中火鸡催乳素调节元件在垂体前叶腺和胰腺中结合的特征和表达
DOI：
10.2141/jpsa.0150091
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
D. Zadworny
中科院分区：
文献类型：
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作者：
Gen Hiyama;N. Kansaku;S. Wakui;Rosanne McQuaid;D. Zadworny
PRL调控元件结合蛋白（regulatory element-binding， PREB）是一种转录因子，最初是从大鼠垂体前叶中克隆出来的，其特征是PRL启动子的调节因子。它也在一些头外组织中强烈表达；然而，它的功能作用至今尚未得到很好的理解。在这项研究中，我们旨在克隆和鉴定火鸡PREB基因，并研究其mRNA在胚胎发生过程中在垂体前叶和胰腺中的表达。基于鸡和哺乳动物PREB cDNA的保守序列，获得了火鸡PREB cDNA片段，对该片段测序后，扩增并确定了mRNA的5′和3′端。采用聚合酶链反应（PCR）扩增方法鉴定PREB基因结构。火鸡PREB基因由9个外显子和8个内含子组成，编码411个氨基酸的蛋白质。在胚胎发生过程中检测垂体前叶PREB mRNA的表达。PREB mRNA水平在胚胎第22天显著升高，在个体发育第25天达到最高水平，这与PRL mRNA水平的变化相似。胰腺中PREB mRNA在第19天达到最高水平。而胰岛素mRNA在胚胎第25天达到最高水平。这些结果表明，PREB可能参与垂体前叶PRL mRNA的表达，而胰岛素mRNA的表达可能独立于胚胎发生过程中胰腺PREB mRNA的表达。
The PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not well understood to date. In this study, we aimed to clone and characterize the turkey PREB gene and investigate its mRNA expression in the anterior pituitary gland and pancreas during embryogenesis. Based on the conserved sequence of chicken and mammalian PREB cDNAs, a turkey PREB cDNA fragment was obtained, and after sequencing of the fragment, the 5′-and 3′-ends of mRNA were amplified and determined. To identify the PREB gene structure, polymerase chain reaction (PCR) amplification was performed. The turkey PREB gene consists of 9 exons and 8 introns, and it encodes a 411-amino-acid protein. The expression of PREB mRNA in the anterior pituitary gland was measured during embryogenesis. Levels of PREB mRNA significantly increased at embryonic day 22, with maximum levels being detected on day 25 of ontogeny, which correlated with similar changes in levels of PRL mRNA. The highest level of PREB mRNA was detected on day 19 in the pancreas. However, the highest level of insulin mRNA was detected at embryonic day 25. These results indicate that PREB may be involved in the expression of PRL mRNA in the anterior pituitary gland, whereas insulin mRNA may be expressed independently of the expression of PREB mRNA in the pancreas during embryogenesis.