Autoimmune response in chronic ongoing myocarditis demonstrated by heterotopic cardiac transplantation in mice.
Autoimmune response in chronic ongoing myocarditis demonstrated by heterotopic cardiac transplantation in mice.
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小鼠异位心脏移植证实慢性持续性心肌炎的自身免疫反应。
DOI：
10.1161/01.cir.94.12.3348
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发表时间：
1996
期刊：
影响因子：
37.8
通讯作者：
M. Matsuzaki
中科院分区：
文献类型：
--
作者：
H. Nakamura;T. Yamamura;S. Umemoto;S. Fukuta;T. Shioi;A. Matsumori;S. Sasayama;M. Matsuzaki
背景
慢性进行性心肌炎的发病机制涉及自身免疫机制。为了研究这种关系，我们使用了一个A/J小鼠模型接种柯萨奇病毒B3，并确定是否心肌炎将转移到异位移植的正常心脏。
方法和结果
用柯萨奇病毒B3（Nancy株; 2 × 10（4）空斑形成单位）腹膜内接种近交的3周龄A/J小鼠并圈养> 60天。在接种后40、50或60天，使用柯萨奇病毒B3基因组5'端特异性引物，通过聚合酶链反应测定心肌中病毒基因组的存在。将正常A/J小鼠心脏移植到同一品系无心肌炎小鼠（A组）和慢性进行性心肌炎小鼠（B组）体内。移植后2周行组织学检查。浸润的T细胞和巨噬细胞进行常规组织学检查，并通过免疫酶染色评价细胞间粘附分子-1、主要组织相容性复合物（MHC）I类抗原和MHC II类抗原的表达。用ELISA法测定移植物中白细胞介素-1 α（IL-1 α）和肿瘤坏死因子（TNF-α）的浓度。在患有慢性心肌炎的小鼠中没有检测到病毒RNA基因组，但它们的移植心脏确实显示出心肌炎。在诱发心肌炎的心脏中，浸润的单核细胞由CD 4 + T细胞、CD 8 + T细胞（CD 4+细胞数量> CD 8+细胞数量）和巨噬细胞组成。细胞间粘附分子-1、MHC I类抗原和MHC II类抗原在浸润病灶内和周围的血管内皮细胞和心肌细胞中表达。B组的IL-1 α和TNF-α浓度显著高于A组（A组与B组：IL-1 α，125 +/- 35 vs 180 +/- 34 pg/mL; TNF-α，45 +/- 15 vs 96 +/- 40 pg/mL; P <0.05）。
结论
结果表明，自身免疫反应可能在慢性进行性心肌炎的进展中发挥关键作用。
BACKGROUND
Autoimmune mechanisms have been implicated in the pathogenesis of chronic ongoing myocarditis. To investigate this relation, we used an A/J mouse model inoculated with coxsackievirus B3 and determined whether myocarditis would be transferred to normal hearts that were heterotopically transplanted.
METHODS AND RESULTS
Inbred 3-week-old A/J mice were inoculated intraperitoneally with coxsackievirus B3 (Nancy strain; 2 x 10(4) plaque-forming units) and housed for > 60 days. The presence of the viral genome in the myocardium was determined by the polymerase chain reaction with primers specific for the 5' end of the coxsackievirus B3 genome performed at 40, 50, or 60 days after inoculation. Normal A/J mouse hearts were transplanted into the same strain of mice without myocarditis (group A) and into mice with chronic ongoing myocarditis (group B). The hearts were evaluated histologically 2 weeks after transplantation. Conventional histological examination of infiltrated T cells and macrophages was performed, and the expression of intercellular adhesion molecule-1, major histocompatibility complex (MHC) class I antigen, and MHC class II antigen was evaluated by immunoenzymatic staining. The concentrations of interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor (TNF-alpha) in the grafts were measured with an ELISA. The viral RNA genomes were not detected in the mice with chronic ongoing myocarditis, but their transplanted hearts did show myocarditis. In the hearts with induced myocarditis, infiltrated mononuclear cells consisted of CD4+ T cells, CD8+ T cells (CD4+ cell number > CD8+ cell number), and macrophages. Intercellular adhesion molecule-1, MHC class I antigen, and MHC class II antigen were expressed in the vascular endothelial cells and myocardial cells in and around the infiltrated lesions. The concentrations of IL-1 alpha and TNF-alpha in group B were significantly higher than those in group A (group A versus group B: IL-1 alpha, 125 +/- 35 versus 180 +/- 34 pg/mL; TNF-alpha, 45 +/- 15 versus 96 +/- 40 pg/mL; P < .05).
CONCLUSIONS
Results suggest that an autoimmune response may play a key role in the progression of chronic ongoing myocarditis.
心肌炎的自身免疫：模型和机制。
DOI：
10.1006/clin.1993.1102
发表时间：
1993
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Rose,NR;Herskowitz,A;Neumann,DA
通讯作者：
Neumann,DA
影响因子：
4.4
作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
通讯作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
影响因子：
4.4
作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer
通讯作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer