Promotion of cell affinity of porous PLLA scaffolds by immobilization of RGD peptides via plasma treatment
Promotion of cell affinity of porous PLLA scaffolds by immobilization of RGD peptides via plasma treatment
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DOI：
10.1002/mabi.200500130
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发表时间：
2006-01-05
影响因子：
4.6
通讯作者：
Wang, DM
中科院分区：
文献类型：
--
作者：
Ho, MH;Hou, LT;Wang, DM
在本工作中，RGDS （Arg-Gly-Asp-Ser）通过血浆处理固定在PLLA支架上。用高效液相色谱法测定固定量，确定固定量的有效顺序。对大鼠成骨肉瘤（ROS）成骨样细胞的培养结果表明，固定化RGDS可有效增强ROS细胞对PLLA的附着，增加PLLA支架中的细胞密度。此外，体外矿化实验表明，与未修饰的PLLA支架相比，rgds固定化支架中细胞和矿化灶更多，有形成骨样组织的倾向。另一方面，用RGES （Arg-Gly-Glu-Ser）固定的PLLA支架促进ROS附着的效果要差得多，这表明细胞附着的增强主要是由于细胞膜上的粘附受体对RGDS的识别。本研究结果表明，通过等离子体处理RGDS可以成功地固定在PLLA支架上，并且这种修饰可以使PLLA支架更适合于培养成骨样细胞和生成骨样组织。
In the present work, RGDS (Arg-Gly-Asp-Ser) was immobilized on PLLA scaffolds with plasma treatment. The amount of immobilization, determined by HPLC, was confirmed to be in the effective order. Results from the culture of rat osteosarcoma (ROS), osteoblastic-like cells, demonstrate that the immobilization of RGDS could effectively enhance the attachment of ROS cells on PLLA and increase the cell density in PLLA scaffolds. In addition, experiments of in vitro mineralization indicate that there were more cells and mineralization focci in the RGDS-immobilized scaffolds, suggesting a tendency to form bone-like tissues, compared with the unmodified PLLA scaffold. On the other hand, the PLLA scaffolds immobilized with RGES (Arg-Gly-Glu-Ser) were much less effective in promotion of ROS attachment, suggesting that the enhancement on cell attachment was mainly due to the recognition of RGDS by the adhesion receptors on the cell membrane. The results presented in this work demonstrate that RGDS could be successfully immobilized on PLLA scaffolds with plasma treatment and such modification can make PLLA scaffolds more suitable for culture of osteoblast-like cells and for generation of bone-like tissues.