Peptide functionalized polyhydroxyalkanoate nanofibrous scaffolds enhance Schwann cells activity.
Peptide functionalized polyhydroxyalkanoate nanofibrous scaffolds enhance Schwann cells activity.
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DOI：
10.1016/j.nano.2014.04.008
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发表时间：
2014-10
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影响因子：
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通讯作者：
E. Masaeli;P. Wieringa;M. Morshed;M. Nasr-Esfahani;Saeid Sadri;C. V. van Blitterswijk;L. Moroni
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文献类型：
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作者：
E. Masaeli;P. Wieringa;M. Morshed;M. Nasr-Esfahani;Saeid Sadri;C. V. van Blitterswijk;L. Moroni
雪旺细胞 (SC) 和支架之间的相互作用对于神经再生过程中的组织发育非常重要，因为 SC 在生理上有助于指导再生轴突的生长。在这项研究中，我们制备了将聚（3-羟基丁酸酯）（PHB）和聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）与胶原蛋白I、H-Gly-Arg-Gly-Asp-Ser-OH（GRGDS）、H-Tyr-Ile-Gly-Ser-Arg-NH2（YIGSR）或H-Arg-Asn-Ile-Ala-Glu-Ile-Ile-Lys-Asp-Ile-OH (p20) 神经模拟肽可模拟天然存在的 ECM 基序以实现神经再生。在呈现这些生物分子的纤维垫上培养的细胞显示出代谢活性和增殖显着增加，同时沿着纤维的方向表现出单向方向。实时 PCR 显示，与未经处理的纳米纤维上培养的细胞相比，在肽修饰支架上培养的细胞具有显着更高的神经营养蛋白表达。我们的研究表明，生物功能化排列的 PHB/PHBV 纳米纤维支架可能会引发 SC 活性的重要线索，并可以作为神经再生的潜在支架。来自临床编辑基于纳米技术的功能化支架代表了周围神经恢复以及脊髓恢复中最有前途的方法之一。在这项研究中，发现生物功能化且对齐的 PHB/PHBV 纳米纤维支架能够引发雪旺细胞活性的重要线索，因此可以作为神经再生的潜在支架。
Interactions between Schwann cells (SCs) and scaffolds are important for tissue development during nerve regeneration, because SCs physiologically assist in directing the growth of regenerating axons. In this study, we prepared electrospun scaffolds combining poly (3-hydroxybutyrate) (PHB) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) functionalized with either collagen I, H-Gly-Arg-Gly-Asp-Ser-OH (GRGDS), H-Tyr-Ile-Gly-Ser-Arg-NH2 (YIGSR), or H-Arg-Asn-Ile-Ala-Glu-Ile-Ile-Lys-Asp-Ile-OH (p20) neuromimetic peptides to mimic naturally occurring ECM motifs for nerve regeneration. Cells cultured on fibrous mats presenting these biomolecules showed a significant increase in metabolic activity and proliferation while exhibiting unidirectional orientation along the orientation of the fibers. Real-time PCR showed cells cultured on peptide-modified scaffolds had a significantly higher neurotrophin expression compared to those on untreated nanofibers. Our study suggests that biofunctionalized aligned PHB/PHBV nanofibrous scaffolds may elicit essential cues for SCs activity and could serve as a potential scaffold for nerve regeneration.From the Clinical EditorNanotechnology-based functionalized scaffolds represent one of the most promising approaches in peripheral nerve recovery, as well as spinal cord recovery. In this study, bio-functionalized and aligned PHB/PHBV nanofibrous scaffolds were found to elicit essential cues for Schwann cell activity, therefore could serve as a potential scaffold for nerve regeneration.