Prolonged upright posture induces degenerative changes in intervertebral discs in rat lumbar spine
Prolonged upright posture induces degenerative changes in intervertebral discs in rat lumbar spine
复制标题
长时间直立姿势引起大鼠腰椎椎间盘退行性变化
DOI：
10.1097/brs.0b013e318183f949
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发表时间：
2008-09-01
期刊：
影响因子：
3
通讯作者：
Wang, Yong-Jun
中科院分区：
文献类型：
--
作者：
Liang, Qian-Qian;Zhou, Quan;Wang, Yong-Jun
研究设计。大鼠的两个前肢被切除，这些大鼠被关在定制的笼子里，让大鼠保持长时间的直立姿势。观察术后不同时间点腰椎的病理变化。探讨直立姿势对大鼠腰椎椎间盘的影响。背景资料摘要。先前的研究表明，脊柱轴向力的增加可以降低椎间盘的高度，但没有数据表明长期和重复的直立姿势是否会导致退行性改变。30只大鼠在1月龄时切除前肢。这些老鼠被关在特制的笼子里，被迫用后腿和尾巴直立起来，以获取水和食物。以常规笼养的同龄正常大鼠为对照。术后5、7、9个月处死大鼠，取腰椎间盘标本进行组织学和免疫组化研究。从这些样品中分离的总RNA用于实时荧光定量PCR检测II型胶原蛋白（Col2 α 1）、X型胶原蛋白（Col10 α 1）、基质金属蛋白酶-13 （MMP-13）、聚集蛋白、崩解素和带血小板反应蛋白基元5的金属蛋白酶（ADAMTS-5）。组织学分析显示，术后椎间盘出现终板软骨胶原结构紊乱、纤维环碎裂、椎间盘高度降低等退行性改变。免疫染色显示ⅱ型胶原蛋白水平降低，X型胶原蛋白表达升高。Real-time PCR结果显示，mmp13、ADAMTS-5和Col10 α 1 mRNA表达上调，Col2 α 1和agrecan mRNA表达下调。长期反复的直立姿势会加速大鼠椎间盘退变。
Study Design. Both forelimbs of rats were amputated, and these rats were kept in the custom-made cages that kept the rats in prolonged upright posture. Pathologic changes were observed in the lumbar spine at different time points after the surgery.Objective. To investigate the effect of upright posture on intervertebral discs of rat lumbar spine.Summary of Background Data. Previous studies have shown that increased axial forces on the spine can decrease the height of the intervertebral disc, but there are no data to indicate whether or not long-term and repeated assumption of the upright posture could result in degenerative changes.Methods. The forelimbs of 30 rats were amputated when they were 1-month old. These rats were kept in the custom-made cages and were forced to stand upright on their hind-limbs and tails to obtain water and food. Normal rats of the same ages kept in regular cages were used as control. The rats were killed at 5, 7, and 9 months after the surgery, and the intervertebral discs samples of lumbar spine were harvested for histologic and immunohistochemical studies. Total RNA isolated from these samples was used for real-time PCR of type II collagen (Col2 alpha 1), type X collagen (Col10 alpha 1), matrix metalloproteinase-13 (MMP-13), aggrecan, and disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5).Results. Histologic analysis showed degenerative changes of the intervertebral discs after surgery such as disordered collagen structure of endplate cartilage, fragmentation of annulus fibrosus, and decreased height of disc. Immunostaining revealed decreased protein level of type II collagen and increased protein expression of type X collagen. Real-time PCR showed upregulated expression of MMP 13, ADAMTS-5, and Col10 alpha 1 mRNA and downregulated mRNA expression of Col2 alpha 1 and aggrecan.Conclusion. Long-term and repeated assumption of the upright stance accelerates disc degeneration in rats.