URB is abundantly expressed in adipose tissue and dysregulated in obesity
URB is abundantly expressed in adipose tissue and dysregulated in obesity
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DOI：
10.1016/j.bbrc.2007.12.164
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发表时间：
2008-03-07
影响因子：
3.1
通讯作者：
Funahashi, Tohru
中科院分区：
文献类型：
--
作者：
Okada, Takuya;Nishizawa, Hitoshi;Funahashi, Tohru
肥胖症患者脂肪细胞因子产生失调与代谢综合征的发生有关。URB/DRO 1含有N端信号序列，被认为在肿瘤细胞凋亡中起作用。在本研究中，我们研究了URB mRNA在脂肪组织和培养的脂肪细胞分泌的表达模式。在人类和小鼠中，URB mRNA主要在脂肪组织中表达，并且在肥胖小鼠模型中下调，例如ob/ob、KKAy和饮食诱导的肥胖小鼠。在3 T3 L1脂肪细胞中，胰岛素、TNF-α、H2 O2和缺氧降低URB mRNA水平。这种调节作用与脂联素相似，与MCP-1相反。URB cDNA稳定转染的细胞培养基中分泌URB蛋白，培养的人脂肪细胞培养基中检测到内源性URB。总之，URB的表达模式表明其在肥胖中的作用，并且结果表明URB至少部分地从脂肪细胞分泌。(c)2007年爱思唯尔公司All rights reserved.
Dysregulated production of adipocytokines in obesity is involved in the development of metabolic syndrome. URB/DRO1 contains N-terminal signal sequence and is thought to play a role in apoptosis of tumor cells. In the present study, we investigated the expression pattern of URB mRNA in adipose tissue and secretion from cultured adipocytes. In human and mouse, URB mRNA was predominantly expressed in adipose tissue and was downregulated in obese mouse models, such as ob/ob, KKAy, and diet-induced obese mice. In 3T3L1 adipocytes, insulin, TNF-alpha, H2O2 and hypoxia decreased URB mRNA level. This regulation was similar to that for adiponectin and opposite to MCP-1. URB protein was secreted in media of URB cDNA-stably transfected cells and endogenous URB was detected in media of cultured human adipocytes. In conclusion, the expression pattern of URB suggests its role in obesity and the results suggest that URB is secreted, at least in part, from adipocytes. (c) 2007 Elsevier Inc. All rights reserved.