Flow kinetics of mouse histocompatibility antigens.
Flow kinetics of mouse histocompatibility antigens.
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小鼠组织相容性抗原的流动动力学。
DOI：
10.1073/pnas.78.3.1547
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发表时间：
1981
影响因子：
11.1
通讯作者：
Morré,DJ
中科院分区：
文献类型：
--
作者：
Croze,EM;Morré,DJ
小鼠的主要组织相容性抗原（H-2抗原）存在于各种不同的细胞类型上，并构成一类参与组织移植物排斥和免疫监视的整合膜结合糖蛋白。我们使用单特异性同种抗体针对小鼠H-2抗原和标准的脉冲追逐技术来研究新合成的膜成分传递到细胞表面的流动动力学。[35 S]甲硫氨酸经腹腔注射，在用未标记的甲硫氨酸追踪后，切除肝脏并分离成内质网、高尔基体和质膜部分。标记首先出现在位于内质网内的H-2抗原中。在标记物注射后5-7 min之间观察到最大比活性，随后标记物快速丢失。高尔基体的H-2抗原也较早被标记。在注射标记物后15-25分钟观察到的峰比活性再次出现，随后标记物迅速丢失。质膜的H-2抗原最后标记，似乎积聚放射性，没有快速转换的证据。这些结果为位于细胞内内质网和高尔基体膜上的H-2抗原与质膜上的H-2抗原之间的抗原-产物关系提供了证据。图中显示了单个膜结合糖蛋白从它们的合成位点和插入内质网膜的位点通过高尔基体到达质膜。
Major histocompatibility antigens of the mouse (H-2 antigens) are found on a variety of different cell types and constitute a class of integral membrane-bound glycoproteins involved in tissue graft rejection and immune surveillance. We used monospecific alloantibodies directed against mouse H-2 antigens and standard pulse-chase technique to investigate the flow kinetics of delivery of newly synthesized membrane constituents to the cell surface. [35S]Methionine was injected intraperitoneally, and, after a chase with unlabeled methionine, livers were excised and fractionated into endoplasmic reticulum, Golgi apparatus, and plasma membrane fractions. Label first appeared in H-2 antigens located within the endoplasmic reticulum. Maximum specific activity observed between 5-7 min after injection of label was followed by a rapid loss of label. H-2 antigens of Golgi apparatus also were labeled early. Peak specific activity observed 15-25 min after injection of label was again followed by rapid loss of label. H-2 antigens of the plasma membrane were labeled last and appeared to accumulate radioactivity with no evidence of rapid turnover. These results provide evidence for a precursor-product relationship between H-2 antigens located within the cell on membranes of endoplasmic reticulum and Golgi apparatus and those on the plasma membrane. Flow of individual membrane-bound glycoproteins from their sites of synthesis and insertion into the membrane of the endoplasmic reticulum to the plasma membrane through the Golgi apparatus is indicated.