High oxygen atmospheric packaging treatment regulates the postharvest changes of Chinese kale (Brassica oleracea var. alboglabra) during storage.
High oxygen atmospheric packaging treatment regulates the postharvest changes of Chinese kale (Brassica oleracea var. alboglabra) during storage.
复制标题
DOI：
10.1111/1750-3841.15846
复制
发表时间：
2021-07
影响因子：
3.9
通讯作者：
Ling Wang;Siliang Wu;Hua Huang;Fei-ping Chen;Mingqiang Ye;Juan Yin;Zheng Luo;Yingwei Qi;Minhui Chen;Yulong Chen
中科院分区：
文献类型：
--
作者：
Ling Wang;Siliang Wu;Hua Huang;Fei-ping Chen;Mingqiang Ye;Juan Yin;Zheng Luo;Yingwei Qi;Minhui Chen;Yulong Chen
芥蓝是中国南方和亚洲最受欢迎的蔬菜之一，但它的保质期很短。以芥蓝为试材，研究了高氧气调包装（HOAP）处理对芥蓝贮藏期间呼吸强度、叶绿素含量及其代谢相关基因表达的影响。结果表明，与对照相比，HOAP处理的芥蓝呼吸速率增加，叶绿素代谢相关基因BrChlases、BrPPH（脱镁叶绿酸水解酶）、BrPAO（pheidea oxygenase gene）、BrRCCR（red chlorophyll catabolite reductase）和BrSAG 12（senescence associated gene）的表达受到调节。HOAP处理对叶绿素酶（Chlase和Mg-脱螯合酶）的活性也有一定的影响。此外，可溶性蛋白质的总含量被刺激积累，和蛋白条带的强度，检测十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，增加HOAP处理的样品。基于目前的研究结果，以及我们以前的研究结果，关于HOAP处理的其他蔬菜，我们推测，HOAP可能通过调节呼吸速率和功能蛋白质的积累，特别是叶绿素分解代谢和抗氧化酶，以保持芥蓝在贮藏期间的新鲜度。实际应用：HOAP处理可延缓芥蓝采后品质变化，延长其货架期。
Chinese kale is one of the most popular vegetables in southern China and Asia, but it has a short shelf-life. The effect of high oxygen atmospheric packaging (HOAP) treatment on the respiration rate as well as chlorophyll content and the expression of their metabolism-related genes of the soluble proteins in Chinese kale during storage were assessed. The results showed that Chinese kale subjected to HOAP treatment showed stimulated respiration rate and regulated expression of chlorophyll metabolism-related genes, such as BrChlases, BrPPH (pheophytin pheophorbide hydrolase), BrPAO (pheidea oxygenase gene), BrRCCR (red chlorophyll catabolite reductase), and BrSAG12 (senescence-associated gene), compared to the Chinese kale in the control. The activities of chlorophyll enzymes, that is, Chlase and Mg-dechelatase, were also influenced by HOAP treatment during storage. Furthermore, the total content of soluble proteins was stimulated to accumulate, and the intensity of protein bands, detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling, increased in HOAP-treated samples. Based on the current results, as well as the results of our previous study regarding HOAP treatment of other vegetables, we speculate that HOAP may function by regulating the respiration rate and the accumulation of functional proteins, especially chlorophyll catabolic and antioxidant enzymes, to maintain the freshness of Chinese kale during storage. PRACTICAL APPLICATION: HOAP treatment could be a potential method for delaying quality changes and extending the shelf-life of Chinese kale after harvest.