Motility and chemotaxis of Pseudomonas sp B4 towards polychlorobiphenyls and chlorobenzoates
Motility and chemotaxis of Pseudomonas sp B4 towards polychlorobiphenyls and chlorobenzoates
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DOI：
10.1111/j.1574-6941.2007.00293.x
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发表时间：
2007-05-01
影响因子：
4.2
通讯作者：
Jerez, Carlos A.
中科院分区：
文献类型：
--
作者：
Gordillo, Felipe;Chavez, Francisco P.;Jerez, Carlos A.
在趋化性试验中，测试了降解多氯联苯（PCB）的假单胞菌B4的运动性和对联苯、其氯代衍生物和氯苯甲酸酯的感知和响应能力。在群板和毛细管测定中，假单胞菌B4被联苯、多氯联苯和苯甲酸酯吸引。对这些化合物的趋化性与它们作为碳和能源的用途有关。在2-和3-氯苯甲酸酯的存在下没有观察到趋化作用。此外，当微生物暴露于3-氯苯甲酸盐时，观察到毒性作用。非运动性假单胞菌B4和异食伯克霍尔德菌LB 400均能以联苯为唯一碳源生长，但与运动性假单胞菌B4相比，其在降解污染物方面存在明显的劣势，说明运动性和趋化性在环境生物降解中的重要性。
The polychlorinated biphenyl (PCB)-degrading Pseudomonas sp. B4 was tested for its motility and ability to sense and respond to biphenyl, its chloroderivatives and chlorobenzoates in chemotaxis assays. Pseudomonas sp. B4 was attracted to biphenyl, PCBs and benzoate in swarm plate and capillary assays. Chemotaxis towards these compounds correlated with their use as carbon and energy sources. No chemotactic effect was observed in the presence of 2- and 3-chlorobenzoates. Furthermore, a toxic effect was observed when the microorganism was exposed to 3-chlorobenzoate. A nonmotile Pseudomonas sp. B4 transformant and Burkholderia xenovorans LB400, the laboratory model strain for PCB degradation, were both capable of growing in biphenyl as the sole carbon source, but showed a clear disadvantage to access the pollutants to be degraded, compared with the highly motile Pseudomonas sp. B4, stressing the importance of motility and chemotaxis in this environmental biodegradation.