Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles
Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles
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DOI：
10.1002/mrd.20205
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发表时间：
2005-03-01
影响因子：
2.5
通讯作者：
Price, CA
中科院分区：
文献类型：
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作者：
Buratini, J;Glapinski, VF;Price, CA
旁分泌细胞信号传导被认为对卵泡发育很重要，并且有人提出了成纤维细胞生长因子 (FGF) 家族的一些成员的作用。在本研究中，我们测试了 FGF-8 及其同源受体（FGFR-3c 和 FGFR-4）在牛窦前卵泡中表达的假设。使用逆转录聚合酶链反应从窦前卵泡样本以及各种胎儿和成人组织中扩增牛 FGF-8、FGFR-3c 和 FGFR-4。所有三个基因在胎儿组织中广泛表达，在成人组织中表达模式有限。 70% 的受检胎儿的次级卵泡中表达 FGF-8 和 FGFR-3c，而 FGFR-4 表达的频率明显较低 (20%)。与次级卵泡相比，原始卵泡中的 FGFR-3c 表达频率显着降低，FGF-8 表达呈现出相似的趋势。仅当个体的所有卵泡类别均表达 FGF-8 和 FGFR-3c 时才观察到 FGFR-4。我们得出结论，FGF-8 及其受体以发育调节方式在窦前卵泡中表达。 (C) 2005 Wiley-Liss, Inc.
Paracrine cell signaling is thought to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family have been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR-3c and FGFR-4) are expressed in bovine preantral follicles. Reverse transcription-polymerase chain reaction was used to amplify bovine FGF-8, FGFR-3c, and FGFR-4 from preantral follicle samples and a variety of fetal and adult tissues. All three genes were widely expressed in fetal tissues, with a restricted expression pattern in adult tissues. FGF-8 and FGFR-3c were expressed in secondary follicles in 70% of fetuses examined, whereas FGFR-4 expression was significantly less frequent (20%). FGFR-3c expression frequency was significantly lower in primordial compared to secondary follicles, and FGF-8 expression showed a similar trend. FGFR-4 was only observed when all follicle classes of an individual were expressing both FGF-8 and FGFR-3c. We conclude that FGF-8 and its receptors are expressed in preantral follicles in a developmentally regulated manner. (C) 2005 Wiley-Liss, Inc.