Effects of butylphthalide on bronchial asthma in guinea pigs and involvement of endothelin
Effects of butylphthalide on bronchial asthma in guinea pigs and involvement of endothelin
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丁苯酞对豚鼠支气管哮喘的影响及内皮素的参与
DOI：
10.13459/j.cnki.cjap.2016.06.006
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Xiao-li CHENG
中科院分区：
文献类型：
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作者：
Zhi-wang WANG;Xiao-yan FU;Yuan REN;Hai-jing DUAN;Xue-feng LIU;Xiao-li CHENG
目的研究丁苯酞对豚鼠支气管哮喘的影响，并探讨内皮素的参与。方法采用注射卵清蛋白（OVA）诱导豚鼠支气管哮喘，吸入卵清蛋白诱发豚鼠支气管哮喘，通过观察卵清蛋白诱导豚鼠肺功能、支气管肺泡灌洗液（BALF）、血清及肺组织中内皮素-1 （ET-1）含量及内皮素转换酶-1 （ECE-1）活性的变化及肺组织中ET-1基因表达，评价丁苯酞对哮喘的影响。结果丁苯酞明显改善肺功能，降低哮喘行为评分，抑制肺组织ECE-1活性，降低肺组织ET-1基因表达水平。结论丁苯酞具有抗哮喘作用，其机制可能与抑制ECE-1活性、降低ET-1geng表达有关。
Objective To study the effects of butylphthalide on bronchial asthma in guinea pigs, and investigate the involvement of endothelin. Methods In guinea pigs, bronchial asthma was induced by injection of ovalbumin (OVA) and provoked by inhalation of OVA, and the effects of butylphthalide on asthma were evaluated through the changes it induced by OVA, pulmonary function, endothelin-1 (ET-1) contents and activity of endothelin converting enzyme-1 (ECE-1) in bronchoalveolar lavage fluid (BALF), serum and lung tissue, and the gene expression of ET-1 in lung tissue. Results Butylphthalide significantly improved pulmonary function, lowered asthmatic behavior score, inhibited the activity of ECE-1, and reduced ET-1 gene expression level in lung tissue. Conclusion Butylphthalide has an anti-asthma effect and the mechanisms involve inhibition of ECE-1 activity and lowering of ET-1geng expression.