LOCALIZATION OF BASEMENT-MEMBRANE COMPONENTS AFTER DERMAL-EPIDERMAL JUNCTION SEPARATION
LOCALIZATION OF BASEMENT-MEMBRANE COMPONENTS AFTER DERMAL-EPIDERMAL JUNCTION SEPARATION
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DOI：
10.1111/1523-1747.ep12543517
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发表时间：
1983-01-01
影响因子：
6.5
通讯作者：
QWARNSTROM, E
中科院分区：
文献类型：
--
作者：
WOODLEY, D;SAUDER, D;QWARNSTROM, E
通过涉及不同作用机制的4种方法在真皮表皮连接处（DEJ）分离成人皮肤：冷1 M盐组织提取、冷胰蛋白酶消化酶促、诱导抽吸水疱机械和温磷酸盐缓冲盐水蛋白酶活化。研究了各种分离方法后DEJ大分子的定位。通过所有测试的方法，大疱性类天疱疮抗原仍然与表皮密切相关，而层粘连蛋白、基底膜硫酸乙酰肝素蛋白聚糖和IV型和V型胶原蛋白仍然与真皮侧分离。大疱性类天疱疮抗原是与表皮基底细胞关系最密切的DEJ成分。基底膜硫酸乙酰肝素蛋白多糖对胰蛋白酶敏感。
Adult human skin was separated at the dermal epidermal junction (DEJ) by 4 methods that involved diffferent mechanism of action : cold 1 M salt tissue extraction cold trypsinization enzymatic, induction of a suction blister mechanical and warm phosphate-buffered saline protease activation. The localiztion of DEJ macromolecules was studied after each separation method. By all of the methods tested, bullous pemphigoid antigen remained closely associated with the epidermis while laminin, the basement membrane heparan sulfate proteoglycan, and collagen types IV and V remained with the dermal side of the separation. The bullous pemphigoid antigen is, then, the DEJ component most closely associated with the epidermal basal cell. Of the basement membrane heparan sulfate proteoglycan was trypsin-sensitive.